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<p>Given the limitations of current IBD treatments, this study conducted an in-depth investigation of 17 human-derived <i>Bifidobacterium longum</i> subsp. <i>longum</i> strains. Primary screening using a lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation model identified strain F05-044-CM-09 as exhibiting significantly stronger inhibition of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α) production compared to other strains. To explore the genetic basis of its anti-inflammatory effect, strain-specific gene analysis revealed that F05-044-CM-09 harbors a gene encoding the CHAP domain, suggesting that anti-inflammatory efficacy may involve immunomodulation through dendritic cell activation and interleukin-2 (IL-2) production. A safety assessment performed prior to animal experiments confirmed the absence of virulence genes and genes encoding D-lactate production. In a dextran sulfate sodium (DSS)-induced murine colitis model, F05-044-CM-09 intervention significantly ameliorated disease phenotypes by mitigating body weight loss and colon shortening, restoring colonic gland architecture, and reducing inflammatory cell infiltration. Analysis of serum biomarkers showed that F05-044-CM-09 significantly reduced LPS levels, whereas serum TNF-α levels showed a decreasing trend that was not statistically significant. However, colonic TNF-α concentrations were significantly reduced following F05-044-CM-09 intervention. In summary, F05-044-CM-09 has the potential to alleviate enteritis and a good safety profile. It is a promising candidate for the development of anti-inflammatory probiotics and offers new possibilities for IBD treatment.</p>

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Screening and Genetic Analysis of Bifidobacterium longum subsp. Longum dipro-X for IBD Alleviation

  • 期刊论文

  • 发表日期：

    2025年10月8日

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  • Yanqing Hao,
  • Wanjin Hu,
  • Weiyang Chen,
  • Ning Xu,
  • Jiang Luo,
  • Weiwei Xiang,
  • Junlin Wu,
  • Wei Jiang,
  • Dongbo Chen,
  • Qinghua Yu

摘要

Given the limitations of current IBD treatments, this study conducted an in-depth investigation of 17 human-derived Bifidobacterium longum subsp. longum strains. Primary screening using a lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation model identified strain F05-044-CM-09 as exhibiting significantly stronger inhibition of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α) production compared to other strains. To explore the genetic basis of its anti-inflammatory effect, strain-specific gene analysis revealed that F05-044-CM-09 harbors a gene encoding the CHAP domain, suggesting that anti-inflammatory efficacy may involve immunomodulation through dendritic cell activation and interleukin-2 (IL-2) production. A safety assessment performed prior to animal experiments confirmed the absence of virulence genes and genes encoding D-lactate production. In a dextran sulfate sodium (DSS)-induced murine colitis model, F05-044-CM-09 intervention significantly ameliorated disease phenotypes by mitigating body weight loss and colon shortening, restoring colonic gland architecture, and reducing inflammatory cell infiltration. Analysis of serum biomarkers showed that F05-044-CM-09 significantly reduced LPS levels, whereas serum TNF-α levels showed a decreasing trend that was not statistically significant. However, colonic TNF-α concentrations were significantly reduced following F05-044-CM-09 intervention. In summary, F05-044-CM-09 has the potential to alleviate enteritis and a good safety profile. It is a promising candidate for the development of anti-inflammatory probiotics and offers new possibilities for IBD treatment.