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<p>In this study, <i>Lacticaseibacillus rhamnosus</i> BELR47 showed the highest lactate production, adhesion to HeLa cells, biofilm formation, and antimicrobial activity against three major bacterial vaginosis (BV)-associated pathogens: <i>Gardnerella vaginalis</i>, <i>Fannyhessea vaginae</i>, and <i>Candida albicans</i>. Moreover, BELR47 exerted anti-inflammatory effects in lipopolysaccharide-induced RAW 264.7 macrophages by suppressing pro-inflammatory cytokine production. In BV-induced mice, oral BELR47 administration significantly reduced vaginal exfoliation and <i>G. vaginalis</i> proliferation compared to the Control group. The Control group exhibited elevated serum interlukin-1β and increased myeloperoxidase, cyclooxygenase 2, inducible nitric oxide synthase, and tumor necrosis factor-alpha mRNA levels in the vagina, cervix, and gut, alongside decreased interlukin-10 expression, which BELR47 treatment reversed. Moreover, <i>G. vaginalis</i> infection diminished colonic expression of tight junction proteins (Claudin-3, Claudin-7, Occludin, ZO-1), indicating impaired gut barrier function; BELR47 ameliorated this dysfunction. BV also reduced fecal microbiome diversity, whereas BELR47 treatment restored it, as reflected by the increased levels of <i>Butyricimonas</i> and <i>Lactobacillus</i> and decreased <i>Parasutterella</i>. Additionally, the elevated butyric acid levels in the BELR47-treated group supported its potential to mitigate gut barrier dysfunction and alleviate intestinal inflammation. Therefore, <i>G. vaginalis</i> infection induces inflammation in both the vagina and colon, disrupts the gut microbiome, and alters fecal metabolites, thereby supporting the concept of a “vagina–gut axis.”</p>

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Lacticaseibacillus rhamnosus BELR47 Mitigates Inflammatory Response in a Gardnerella vaginalis-Induced Bacterial Vaginosis Mouse Model via Gut Microbiome Modulation

  • 期刊论文

  • 发表日期：

    2025年10月27日

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  • Hyun Jin Bae,
  • Dong Hwan Lee,
  • Hyo Su Choi,
  • Hye Ji Lim,
  • Eui-Chun Chung,
  • Nam Su Oh

摘要

In this study, Lacticaseibacillus rhamnosus BELR47 showed the highest lactate production, adhesion to HeLa cells, biofilm formation, and antimicrobial activity against three major bacterial vaginosis (BV)-associated pathogens: Gardnerella vaginalis, Fannyhessea vaginae, and Candida albicans. Moreover, BELR47 exerted anti-inflammatory effects in lipopolysaccharide-induced RAW 264.7 macrophages by suppressing pro-inflammatory cytokine production. In BV-induced mice, oral BELR47 administration significantly reduced vaginal exfoliation and G. vaginalis proliferation compared to the Control group. The Control group exhibited elevated serum interlukin-1β and increased myeloperoxidase, cyclooxygenase 2, inducible nitric oxide synthase, and tumor necrosis factor-alpha mRNA levels in the vagina, cervix, and gut, alongside decreased interlukin-10 expression, which BELR47 treatment reversed. Moreover, G. vaginalis infection diminished colonic expression of tight junction proteins (Claudin-3, Claudin-7, Occludin, ZO-1), indicating impaired gut barrier function; BELR47 ameliorated this dysfunction. BV also reduced fecal microbiome diversity, whereas BELR47 treatment restored it, as reflected by the increased levels of Butyricimonas and Lactobacillus and decreased Parasutterella. Additionally, the elevated butyric acid levels in the BELR47-treated group supported its potential to mitigate gut barrier dysfunction and alleviate intestinal inflammation. Therefore, G. vaginalis infection induces inflammation in both the vagina and colon, disrupts the gut microbiome, and alters fecal metabolites, thereby supporting the concept of a “vagina–gut axis.”