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<p>This study evaluated the probiotic characteristics of <i>Lactiplantibacillus plantarum</i> strains, revealing strong acid and bile salt tolerance and significant adhesion to HT-29 cells. In addition, the anti-melanogenic and antioxidant properties of their cell-free supernatants (CFS) were investigated in vitro. Melanogenesis assays were performed in α-melanocyte-stimulating hormone (α-MSH)-stimulated B16F10 melanoma cells, while antioxidant activity was evaluated in H<sub>2</sub>O<sub>2</sub>-stimulated HaCaT keratinocytes. CFS treatment inhibited melanin synthesis, tyrosinase activity, and expression of melanogenesis-related genes, including <i>MITF</i>, <i>Tyr</i>, <i>Trp1</i>, and <i>Trp2</i>. These effects were attributed to the suppression of cAMP-response element-binding protein (CREB)/microphthalmia-associated transcription factor (MITF) and mitogen-activated protein kinase (MAPK; ERK, JNK, and p38) signaling pathways. Antioxidant activity was observed via nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway activation, resulting in elevated levels of downstream targets such as HO-1, NAD(P)H:quinone oxidoreductase (NQO1), and thioredoxin reductase 1 (TXNRD1). High-performance liquid chromatography (HPLC) analysis identified lactic acid and acetic acid as the primary metabolites in the CFS associated with these biological effects. These findings suggest that culture supernatants derived from probiotics may serve as natural agents with skin-whitening and antioxidant properties, supporting their potential application in food, pharmaceutical, and cosmetic industries.</p>

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Anti-melanogenic and Antioxidant Activities of Lactiplantibacillus plantarum Strains in Skin Cells via the CREB/MITF and Nrf2/HO-1 Pathways

  • 期刊论文

  • 发表日期：

    2025年7月30日

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  • Seung-Yeon Cha,
  • Im-Kyung Woo,
  • Yu-Jin Cha,
  • Na-Kyoung Lee,
  • Hye Ji Jang,
  • Hyun-Dong Paik

摘要

This study evaluated the probiotic characteristics of Lactiplantibacillus plantarum strains, revealing strong acid and bile salt tolerance and significant adhesion to HT-29 cells. In addition, the anti-melanogenic and antioxidant properties of their cell-free supernatants (CFS) were investigated in vitro. Melanogenesis assays were performed in α-melanocyte-stimulating hormone (α-MSH)-stimulated B16F10 melanoma cells, while antioxidant activity was evaluated in H2O2-stimulated HaCaT keratinocytes. CFS treatment inhibited melanin synthesis, tyrosinase activity, and expression of melanogenesis-related genes, including MITF, Tyr, Trp1, and Trp2. These effects were attributed to the suppression of cAMP-response element-binding protein (CREB)/microphthalmia-associated transcription factor (MITF) and mitogen-activated protein kinase (MAPK; ERK, JNK, and p38) signaling pathways. Antioxidant activity was observed via nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway activation, resulting in elevated levels of downstream targets such as HO-1, NAD(P)H:quinone oxidoreductase (NQO1), and thioredoxin reductase 1 (TXNRD1). High-performance liquid chromatography (HPLC) analysis identified lactic acid and acetic acid as the primary metabolites in the CFS associated with these biological effects. These findings suggest that culture supernatants derived from probiotics may serve as natural agents with skin-whitening and antioxidant properties, supporting their potential application in food, pharmaceutical, and cosmetic industries.