Scholarlink

<p>A gene encoding endolysin LysEf-9 from the phage PEF9, a lytic enterococcal phage, was cloned and overexpressed in <i>Escherichia coli</i>. The expressed LysEf-9 protein with a concentration of 62.5&#xa0;μg/mL reduced the turbidity and viability of <i>E. faecalis</i> JCM 7783 after 3&#xa0;h of incubation. LysEf-9 showed stability at different pH (2–12) and withstood different temperature treatments up to 60&#xa0;°C. In addition, it showed a wide spectrum against <i>Enterococci.</i> Phage vB_EfKS5 with antibacterial and antibiofilm properties against <i>E. faecalis</i> was isolated previously. The phage-resistant mutant was isolated and used as an indicator strain in the present study. A bacteriocin-producing <i>E. faecalis</i> strain was also isolated, and the cell-free supernatant (crude bacteriocin) was partially purified by ammonium sulfate precipitation. The crude bacteriocin showed a wide spectrum against <i>Enterococcus</i> spp. and lysed the phage-resistant mutant. Using LysEf-9 with the crude bacteriocin reduced the viable count of <i>E. faecalis</i> JCM 3378 by 2.45 log after 24&#xa0;h. In addition, LysEf-9 with phage vB_EfKS5 retarded the growth of the phage-resistant mutant. The antibiofilm efficacy of LysEf-9 and crude bacteriocin was also determined. LysEf-9 lowered the viable count of <i>E. faecalis</i> JCM 7783, <i>E. faecium</i> No. 26, and the phage-resistant mutant in the 48-h biofilm by 3 log, 2 log, and 1.5 log, respectively. The combination of LysEf-9 with crude bacteriocin reduced the total viable count of the indicator strains by at least 3 logs. This study shows the strong antibacterial and antibiofilm activity of LysEf-9, and the activity was enhanced when used with crude bacteriocin or phage.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Antibacterial and Antibiofilm Efficacy of Endolysin LysEf-9 and Crude Bacteriocin Against Multidrug and Phage-Resistant Enterococci

  • 期刊论文

  • 发表日期：

    2025年6月7日

查看全文
  • Mohamed El-Telbany,
  • Chen Wang,
  • Martha Clokie,
  • Su Zar Chi Lwin,
  • Marwa Nabil Sayed Abdelaziz,
  • Aye Thida Maung,
  • Khin Zar Linn,
  • Catherine Hofilena Damaso,
  • Yunzhi Lin,
  • Haomin Ye,
  • Yoshimitsu Masuda,
  • Ken-ichi Honjoh,
  • Takahisa Miyamoto

摘要

A gene encoding endolysin LysEf-9 from the phage PEF9, a lytic enterococcal phage, was cloned and overexpressed in Escherichia coli. The expressed LysEf-9 protein with a concentration of 62.5 μg/mL reduced the turbidity and viability of E. faecalis JCM 7783 after 3 h of incubation. LysEf-9 showed stability at different pH (2–12) and withstood different temperature treatments up to 60 °C. In addition, it showed a wide spectrum against Enterococci. Phage vB_EfKS5 with antibacterial and antibiofilm properties against E. faecalis was isolated previously. The phage-resistant mutant was isolated and used as an indicator strain in the present study. A bacteriocin-producing E. faecalis strain was also isolated, and the cell-free supernatant (crude bacteriocin) was partially purified by ammonium sulfate precipitation. The crude bacteriocin showed a wide spectrum against Enterococcus spp. and lysed the phage-resistant mutant. Using LysEf-9 with the crude bacteriocin reduced the viable count of E. faecalis JCM 3378 by 2.45 log after 24 h. In addition, LysEf-9 with phage vB_EfKS5 retarded the growth of the phage-resistant mutant. The antibiofilm efficacy of LysEf-9 and crude bacteriocin was also determined. LysEf-9 lowered the viable count of E. faecalis JCM 7783, E. faecium No. 26, and the phage-resistant mutant in the 48-h biofilm by 3 log, 2 log, and 1.5 log, respectively. The combination of LysEf-9 with crude bacteriocin reduced the total viable count of the indicator strains by at least 3 logs. This study shows the strong antibacterial and antibiofilm activity of LysEf-9, and the activity was enhanced when used with crude bacteriocin or phage.