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<p>Skin aging is influenced by structural alterations, oxidative stress, inflammation, and microbiome changes, and a comprehensive approach to addressing these factors may be effective for mitigating skin aging. This study evaluates the multifaceted anti-aging effects of heat-killed (HK-HN910) and lysed (LS-HN910) forms of <i>Lactobacillus paragasseri</i> HN910. Protective effects on cell viability, cell permeability, nitric oxide (NO) production, and skin anti-aging gene expression for both HK-HN910 and LS-HN910 were observed. Both forms significantly enhanced tight junction (TJ) protein zonula occludens- 1 (<i>ZO- 1</i>) and antioxidant enzyme glutathione peroxidase (<i>GPx)</i> gene expression, while significantly downregulating that of senescence-associated secretory phenotype pro-inflammatory cytokines interleukin (<i>IL</i>)<i>- 1α</i>, <i>IL- 1β</i>, <i>IL- 6</i>, <i>IL- 8</i>, and tumor necrosis factor-alpha (<i>TNFα</i>). LS-HN910 showed significantly greater upregulation of <i>ZO- 1</i> and <i>GPx</i> and greater downregulation of <i>IL- 1β</i> and <i>TNFα</i> expression compared to HK-HN910. Cell wall component D-alanine (D-Ala) was released in higher amounts in LS-HN910 than in HK-HN910 and demonstrated anti-aging effects. D-Ala upregulated gene expression of skin barrier <i>ZO- 1</i>, claudin- 1 (<i>Cla- 1</i>), occludin (<i>OCC</i>), filaggrin (<i>FLG</i>), and sphingomyelin phosphodiesterase 2 (<i>SMPD2</i>) and antioxidant enzymes superoxide dismutase (<i>SOD</i>), catalase (<i>CAT</i>), and <i>GPx</i>, while downregulating <i>IL- 1α</i>, <i>IL- 1β</i>, <i>IL- 6</i>, <i>IL- 8</i>, and <i>TNFα</i>. LS-HN910 treatment clinically revealed improvements in anti-aging parameters, including transepidermal water loss, skin water contents, sebum levels, dermal density, eye wrinkle index, skin pH, brightness, and microbiota composition, with a significant increase in <i>Rhodococcus</i> abundance. These findings indicate that LS-HN910, containing released D-Ala, is a promising cosmeceutical for preventing skin aging by enhancing the skin barrier, promoting oxidative defense, modulating inflammatory responses, and influencing skin microbiota.</p>

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Skin Protection Effects of Lactobacillus paragasseri HN910 Lysate and the Role of Alanine

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年4月17日

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  • Chaewon Lee,
  • Lisong Pei,
  • Hyunjun Park,
  • Heebal Kim,
  • Chul Sung Huh

摘要

Skin aging is influenced by structural alterations, oxidative stress, inflammation, and microbiome changes, and a comprehensive approach to addressing these factors may be effective for mitigating skin aging. This study evaluates the multifaceted anti-aging effects of heat-killed (HK-HN910) and lysed (LS-HN910) forms of Lactobacillus paragasseri HN910. Protective effects on cell viability, cell permeability, nitric oxide (NO) production, and skin anti-aging gene expression for both HK-HN910 and LS-HN910 were observed. Both forms significantly enhanced tight junction (TJ) protein zonula occludens- 1 (ZO- 1) and antioxidant enzyme glutathione peroxidase (GPx) gene expression, while significantly downregulating that of senescence-associated secretory phenotype pro-inflammatory cytokines interleukin (IL)- 1α, IL- 1β, IL- 6, IL- 8, and tumor necrosis factor-alpha (TNFα). LS-HN910 showed significantly greater upregulation of ZO- 1 and GPx and greater downregulation of IL- 1β and TNFα expression compared to HK-HN910. Cell wall component D-alanine (D-Ala) was released in higher amounts in LS-HN910 than in HK-HN910 and demonstrated anti-aging effects. D-Ala upregulated gene expression of skin barrier ZO- 1, claudin- 1 (Cla- 1), occludin (OCC), filaggrin (FLG), and sphingomyelin phosphodiesterase 2 (SMPD2) and antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and GPx, while downregulating IL- 1α, IL- 1β, IL- 6, IL- 8, and TNFα. LS-HN910 treatment clinically revealed improvements in anti-aging parameters, including transepidermal water loss, skin water contents, sebum levels, dermal density, eye wrinkle index, skin pH, brightness, and microbiota composition, with a significant increase in Rhodococcus abundance. These findings indicate that LS-HN910, containing released D-Ala, is a promising cosmeceutical for preventing skin aging by enhancing the skin barrier, promoting oxidative defense, modulating inflammatory responses, and influencing skin microbiota.