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<p>Okadaic acid (OA), a significant factor contributing to the occurrence of diarrheic shellfish poisoning (DSP), bioaccumulates in shellfish along the food chain, and the influx of shellfish products containing this toxin into the market can seriously affect human health. Therefore, a rapid and accurate method for OA analysis and field detection must be established. In this study, time-resolved fluorescence analysis and immunochromatographic techniques were combined to establish a time-resolved fluorescence immunochromatographic (TRFICA) technique. The use of europium microspheres coupled with OA monoclonal antibody as an indicator signal allows for the competitive binding of OA antigens, thereby enabling the quantitative detection of the OA concentration. The limit of detection of the method established in this study was 0.24&#xa0;ng/mL, the sensitivity was 0.16&#xa0;ng/mL, the limit of quantification was 1.83&#xa0;ng/mL, the precision ranged from 6.86 to 8.17%, and the recoveries ranged from 108.00 to 117.52%. TRFICA and high-performance liquid chromatography were used to determine the OA content in the shellfish samples, and there is a relatively good correlation (<i>y</i> = 0.6919<i>x</i> + 2.9564, <i>R</i><sup>2</sup> = 0.6667, <i>p</i> &lt; 0.05). The TRFICA method was established as a swift and preciseonsite assay for the detection of OA, and it is expected to contribute to the detection of shellfish toxins in the future.</p>

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Time-Resolved Fluorescence Immunochromatography Sensor for Detecting Okadaic Acid in Shellfish

  • 期刊论文

  • 发表日期：

    2025年4月4日

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  • Zhi Zhang,
  • Yaofan Lu,
  • Fuyuan Ma,
  • Chenhao Zhao,
  • Jiayu Li,
  • Biao Huang,
  • Yuan Qin

摘要

Okadaic acid (OA), a significant factor contributing to the occurrence of diarrheic shellfish poisoning (DSP), bioaccumulates in shellfish along the food chain, and the influx of shellfish products containing this toxin into the market can seriously affect human health. Therefore, a rapid and accurate method for OA analysis and field detection must be established. In this study, time-resolved fluorescence analysis and immunochromatographic techniques were combined to establish a time-resolved fluorescence immunochromatographic (TRFICA) technique. The use of europium microspheres coupled with OA monoclonal antibody as an indicator signal allows for the competitive binding of OA antigens, thereby enabling the quantitative detection of the OA concentration. The limit of detection of the method established in this study was 0.24 ng/mL, the sensitivity was 0.16 ng/mL, the limit of quantification was 1.83 ng/mL, the precision ranged from 6.86 to 8.17%, and the recoveries ranged from 108.00 to 117.52%. TRFICA and high-performance liquid chromatography were used to determine the OA content in the shellfish samples, and there is a relatively good correlation (y = 0.6919x + 2.9564, R2 = 0.6667, p < 0.05). The TRFICA method was established as a swift and preciseonsite assay for the detection of OA, and it is expected to contribute to the detection of shellfish toxins in the future.