Scholarlink

<p><i>Laureliopsis philippiana</i> (Looser) Schodde commonly known as “Tepa”, is an aromatic evergreen tree from Patagonia considered a valuable timber resource due to its versatility. The essential oil (EO) from the leaves and bark of <i>L. philippiana</i> exhibits strong insecticidal activity against pests of agricultural interest. However, their use in integrated pest management (IPM) should take into account their potential toxicity to pollinating insects. Therefore, this study evaluated the tixicity of <i>L. philippiana</i> EO and its nanoemulsion (NE) formulation on the non-target pollinator <i>Bombus terrestris</i> L. The EO was chemically characterized by GC–MS, identifying linalool (38.1%), safrole (28.2%), and 1,8-cineole (12.8%) as the major constituents. Nanoemulsification preserved the chemical profile of the EO while yielding a stable formulation with droplet sizes below 50 nm and sustained physicochemical stability over 35 days. Fumigation assays showed that the essential oil exhibited dose- and time-dependent toxicity, with LC₅₀ values of 607.7 at 4 h, 189.7 at 8 h, and 31.7 µL L⁻<sup>1</sup> at 48 h. The nanoemulsion was effective even at lower concentrations, reaching LC₅₀ values of 77.6 at 24 h and 63.3 µL L⁻<sup>1</sup> at 48 h, indicating prolonged insecticidal activity. Single-dose fumigation revealed a faster action for the pure oil (LT₅₀ = 10.9; LT₉₀ = 34.7 h) compared to the nanoemulsion (LT₅₀ = 29.0; LT₉₀ = 157.9 h). Topical contact bioassays confirmed rapid lethality of the pure oil (LD₅₀ = 5.2 µL at 4 h), whereas the nanoemulsion maintained stable activity over 24 h (LD₅₀ = 6.3 µL). These findings suggest that, although the essential oil acts more rapidly, the nanoemulsion provides sustained bioactivity, likely due to controlled-release and improved compound dispersion. Both formulations exhibited significant toxicity against <i>B. terrestris</i>, highlighting the need for careful dose and exposure-time management when considering potential field applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Acute toxicity of Laureliopsis philippiana essential oil and its nanoemulsion to Bombus terrestris

  • 期刊论文

  • 发表日期：

    2026年7月24日

查看全文
  • Braulio Cedeño Vera,
  • María Dolores López,
  • Nolberto Arismendi,
  • Marisol Vargas,
  • Daniel A. Palacio,
  • Ana María Salazar,
  • María Eugenia Romero,
  • Diego Portalanza,
  • Guy Smagghe,
  • Nelson Zapata

摘要

Laureliopsis philippiana (Looser) Schodde commonly known as “Tepa”, is an aromatic evergreen tree from Patagonia considered a valuable timber resource due to its versatility. The essential oil (EO) from the leaves and bark of L. philippiana exhibits strong insecticidal activity against pests of agricultural interest. However, their use in integrated pest management (IPM) should take into account their potential toxicity to pollinating insects. Therefore, this study evaluated the tixicity of L. philippiana EO and its nanoemulsion (NE) formulation on the non-target pollinator Bombus terrestris L. The EO was chemically characterized by GC–MS, identifying linalool (38.1%), safrole (28.2%), and 1,8-cineole (12.8%) as the major constituents. Nanoemulsification preserved the chemical profile of the EO while yielding a stable formulation with droplet sizes below 50 nm and sustained physicochemical stability over 35 days. Fumigation assays showed that the essential oil exhibited dose- and time-dependent toxicity, with LC₅₀ values of 607.7 at 4 h, 189.7 at 8 h, and 31.7 µL L⁻1 at 48 h. The nanoemulsion was effective even at lower concentrations, reaching LC₅₀ values of 77.6 at 24 h and 63.3 µL L⁻1 at 48 h, indicating prolonged insecticidal activity. Single-dose fumigation revealed a faster action for the pure oil (LT₅₀ = 10.9; LT₉₀ = 34.7 h) compared to the nanoemulsion (LT₅₀ = 29.0; LT₉₀ = 157.9 h). Topical contact bioassays confirmed rapid lethality of the pure oil (LD₅₀ = 5.2 µL at 4 h), whereas the nanoemulsion maintained stable activity over 24 h (LD₅₀ = 6.3 µL). These findings suggest that, although the essential oil acts more rapidly, the nanoemulsion provides sustained bioactivity, likely due to controlled-release and improved compound dispersion. Both formulations exhibited significant toxicity against B. terrestris, highlighting the need for careful dose and exposure-time management when considering potential field applications.