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<p><i>Orobanche crenata</i> (crenate broomrape) is a devastating parasitic weed that severely constrains legume and vegetable production in the Mediterranean region. In Morocco, where it is particularly destructive to faba bean, chickpea, and more recently carrot, little is known about its genetic structure and host adaptation. To investigate its genetic structure and host-associated differentiation, we characterized 45 populations of <i>O. crenata</i> collected from these three hosts using 11 ISSR primers. A total of 217 scorable bands were obtained, with a high level of polymorphism (99.07%). Marker informativeness was supported by high polymorphism information content (mean = 0.34) and resolving power (mean = 6.85). Genetic diversity indices revealed moderate variation within populations (I = 0.32; Ht = 0.19), while AMOVA indicated that 23.2% of the total variance was attributable to differences among populations. Pairwise comparisons showed the strongest differentiation between carrot and legume-associated populations (FST = 0.12; Nei’s distance = 0.029), whereas faba bean and chickpea populations were more closely related (FST = 0.075; Nei’s distance = 0.020). These findings, further supported by NJ clustering and PCoA, demonstrate that host identity plays a central role in structuring <i>O. crenata</i> populations in Morocco. This study provides the first molecular evidence of host-driven genetic divergence in carrot-derived <i>O. crenata</i> and highlights the importance of considering host specialization in breeding programs and management strategies.</p>

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Genetic characterization of Orobanche crenata populations parasitizing faba bean, chickpea, and carrot in Morocco reveals host-driven differentiation

  • 期刊论文

  • 发表日期：

    2026年2月13日

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  • Toufik Chedadi,
  • Youssef Khachtib,
  • Youssef Ait Bella,
  • Atman Adiba,
  • Said Bouda,
  • Abdelmajid Haddioui,
  • Mohammed El Hansali,
  • Omar Idrissi

摘要

Orobanche crenata (crenate broomrape) is a devastating parasitic weed that severely constrains legume and vegetable production in the Mediterranean region. In Morocco, where it is particularly destructive to faba bean, chickpea, and more recently carrot, little is known about its genetic structure and host adaptation. To investigate its genetic structure and host-associated differentiation, we characterized 45 populations of O. crenata collected from these three hosts using 11 ISSR primers. A total of 217 scorable bands were obtained, with a high level of polymorphism (99.07%). Marker informativeness was supported by high polymorphism information content (mean = 0.34) and resolving power (mean = 6.85). Genetic diversity indices revealed moderate variation within populations (I = 0.32; Ht = 0.19), while AMOVA indicated that 23.2% of the total variance was attributable to differences among populations. Pairwise comparisons showed the strongest differentiation between carrot and legume-associated populations (FST = 0.12; Nei’s distance = 0.029), whereas faba bean and chickpea populations were more closely related (FST = 0.075; Nei’s distance = 0.020). These findings, further supported by NJ clustering and PCoA, demonstrate that host identity plays a central role in structuring O. crenata populations in Morocco. This study provides the first molecular evidence of host-driven genetic divergence in carrot-derived O. crenata and highlights the importance of considering host specialization in breeding programs and management strategies.