Scholarlink

<p>This study evaluated the efficacy of thermotherapy and ozone treatments in inactivating the seed-transmitted viruses in chickpea (<i>Cicer arietinum</i> L.) and lentil (<i>Lens culinaris</i> L.) seeds. Targeted viruses included Alfalfa mosaic virus (<i>Alfamovirus AMV</i>, AMV), Bean yellow mosaic virus (<i>Potyvirus phaseoluteum</i>, BYMV), Cucumber mosaic virus (<i>Cucumovirus CMV</i>, CMV), and Pea seed-borne mosaic virus (<i>Potyvirus pisumsemenportati</i>, PSbMV). Seeds were treated with hot water (HW) (55&#xa0;°C for 15, 20, 25&#xa0;min), dry heat (DH) (60, 70, 80&#xa0;°C for 1&#xa0;h), and ozone (O<sub>3</sub>) (ozonated water at 8&#xa0;g/Nm<sup>3</sup>, ozone gas at 100&#xa0;g/Nm<sup>3</sup> for 10, 30, 60&#xa0;min on dry or pre-soaked seeds). Virus concentrations were measured via DAS-ELISA, while percent germination (PG) and mean germination time (MGT) were used as seed quality indicators. Ozone treatments, especially ozonated water, achieved complete virus inactivation (up to 100%) and maintained high germination rates (up to 100%) in several virus-seed combinations, including PSbMV in chickpea and BYMV in lentil. Dry heat at 60&#xa0;°C was similarly effective but showed reduced normal germination at higher temperatures. In contrast, hot water treatments significantly impaired seed viability, with percent germination dropping to 6% in lentils and increasing abnormal germination. Ozone treatments had no adverse effects and even enhanced radicle and root development. Among all methods, ozonated water emerged as the most promising non-chemical treatment for virus inactivation without compromising seed quality. These findings suggest its potential for improving seed health in sustainable legume production systems.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Effects of ozone treatments and thermotherapy on the inactivation of several seed-borne viruses in chickpea (Cicer arietinum L.) and lentil (Lens culinaris L.) seeds

  • 期刊论文

  • 发表日期：

    2025年8月1日

查看全文
  • Mehmet Zeki Kızmaz,
  • Mustafa Gümüş

摘要

This study evaluated the efficacy of thermotherapy and ozone treatments in inactivating the seed-transmitted viruses in chickpea (Cicer arietinum L.) and lentil (Lens culinaris L.) seeds. Targeted viruses included Alfalfa mosaic virus (Alfamovirus AMV, AMV), Bean yellow mosaic virus (Potyvirus phaseoluteum, BYMV), Cucumber mosaic virus (Cucumovirus CMV, CMV), and Pea seed-borne mosaic virus (Potyvirus pisumsemenportati, PSbMV). Seeds were treated with hot water (HW) (55 °C for 15, 20, 25 min), dry heat (DH) (60, 70, 80 °C for 1 h), and ozone (O3) (ozonated water at 8 g/Nm3, ozone gas at 100 g/Nm3 for 10, 30, 60 min on dry or pre-soaked seeds). Virus concentrations were measured via DAS-ELISA, while percent germination (PG) and mean germination time (MGT) were used as seed quality indicators. Ozone treatments, especially ozonated water, achieved complete virus inactivation (up to 100%) and maintained high germination rates (up to 100%) in several virus-seed combinations, including PSbMV in chickpea and BYMV in lentil. Dry heat at 60 °C was similarly effective but showed reduced normal germination at higher temperatures. In contrast, hot water treatments significantly impaired seed viability, with percent germination dropping to 6% in lentils and increasing abnormal germination. Ozone treatments had no adverse effects and even enhanced radicle and root development. Among all methods, ozonated water emerged as the most promising non-chemical treatment for virus inactivation without compromising seed quality. These findings suggest its potential for improving seed health in sustainable legume production systems.