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<p><i>Diaphania indica</i> (Saunders) (Lepidoptera: Crambidae) is an important pest of snake gourd, <i>Trichosanthes anguina</i> (L.). A comparative analysis of Chlorophyll <i>a</i> and <i>b</i>, total chlorophyll, proteins, phenols, reactive oxygen species (ROS), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), malondialdehyde (MDA) and antioxidative enzymes [catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), ascorbate peroxidase (APOX), pyrogallol peroxidase (PPX) and guaiacol peroxidase (GPX)] of three snake gourd cultivars, MNSR-1 (MNS), Baruipur Long (BAR) and Polo No. 1 (POLO) after 4 or 12&#xa0;h of <i>D. indica</i> feeding were conducted to observe the induced defense mechanism of these cultivars (<i>n</i> = 5). 3,3ʹ-Diaminobenzidine (DAB) and nitro blue tetrazolium (NBT) staining, and expression of <i>TaCAT3</i> gene were observed in undamaged and insect-damaged cultivars (<i>n</i> = 3). Chlorophyll <i>a</i> and <i>b</i>, total chlorophyll and proteins decreased with increased feeding time in each cultivar. Total ROS, H<sub>2</sub>O<sub>2</sub>, MDA, phenols and antioxidative enzymes were enhanced with increased feeding time in each cultivar. After 12&#xa0;h of feeding, DAB staining was 5.43 and 8.71% higher in POLO than MNS and BAR (<i>P</i> &lt; 0.05), respectively, whereas NBT staining was 8.68 and 30.22% higher in POLO than MNS and BAR (<i>P</i> &lt; 0.05), respectively. In contrast, significant increases in activities of enzymatic antioxidants (CAT, PPX and GPX) and total phenols were observed in POLO than MNS and BAR (<i>P</i> &lt; 0.05). After 12&#xa0;h of insect feeding, induction of <i>TaCAT3</i> gene was significantly higher in POLO than MNS (<i>P</i> &lt; 0.05). This study concludes that MNS and BAR were more susceptible to <i>D. indica</i> than POLO.</p>

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Resistance of Trichosanthes anguina (L.) cultivars due to herbivory by caterpillars of Diaphania indica (Saunders) (Lepidoptera: Crambidae)

  • 期刊论文

  • 发表日期：

    2025年4月22日

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  • Rahul Debnath,
  • Susmita Das,
  • Sanoj Kumbhakar,
  • Bhramar Bhattacharyya,
  • Sayantan Dutta,
  • Anandamay Barik

摘要

Diaphania indica (Saunders) (Lepidoptera: Crambidae) is an important pest of snake gourd, Trichosanthes anguina (L.). A comparative analysis of Chlorophyll a and b, total chlorophyll, proteins, phenols, reactive oxygen species (ROS), hydrogen peroxide (H2O2), malondialdehyde (MDA) and antioxidative enzymes [catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), ascorbate peroxidase (APOX), pyrogallol peroxidase (PPX) and guaiacol peroxidase (GPX)] of three snake gourd cultivars, MNSR-1 (MNS), Baruipur Long (BAR) and Polo No. 1 (POLO) after 4 or 12 h of D. indica feeding were conducted to observe the induced defense mechanism of these cultivars (n = 5). 3,3ʹ-Diaminobenzidine (DAB) and nitro blue tetrazolium (NBT) staining, and expression of TaCAT3 gene were observed in undamaged and insect-damaged cultivars (n = 3). Chlorophyll a and b, total chlorophyll and proteins decreased with increased feeding time in each cultivar. Total ROS, H2O2, MDA, phenols and antioxidative enzymes were enhanced with increased feeding time in each cultivar. After 12 h of feeding, DAB staining was 5.43 and 8.71% higher in POLO than MNS and BAR (P < 0.05), respectively, whereas NBT staining was 8.68 and 30.22% higher in POLO than MNS and BAR (P < 0.05), respectively. In contrast, significant increases in activities of enzymatic antioxidants (CAT, PPX and GPX) and total phenols were observed in POLO than MNS and BAR (P < 0.05). After 12 h of insect feeding, induction of TaCAT3 gene was significantly higher in POLO than MNS (P < 0.05). This study concludes that MNS and BAR were more susceptible to D. indica than POLO.