Nanoparticle Enhanced Laser Induced Breakdown Spectroscopy (NELIBS) Analysis Of Human Teeth By Using ZnO And Al2O3 Nanoparticles
摘要
Nanotechnology is a rapidly growing field in material science, particularly in dental care. It holds great potential in tissue repair and replacement, particularly in oral mineralized tissues. Zinc oxide nanoparticles (ZnO NPs) and alumina nanoparticles (Al2O3 NPs) have emerged as promising tools for diagnostics and therapeutics in biomedicine. These nanoparticles have antibacterial, regenerative, and mechanical actions. They can also enhance laser-induced breakdown spectroscopy (LIBS) by adding a specific nanoparticle to human dental enamel surface. A study was conducted to evaluate the practicality of using modified surface-enhanced human teeth substrates for quantitative NELIBS. Various types of nanoparticles were used to build tooth substrates with varied tooth surface coverage. The study found that the use of a 1064 nm laser wavelength and 200 mJ energy/pulse resulted in the most effective enhancement of the NELIBS signal, particularly when treated with ZnO NPs. Quantitative analysis was determined using Calibration-Curve laser-induced breakdown spectroscopy (CC-LIBS). Enhanced signal emission was observed using the Ca II spectral line at 396.84 nm after adding nanoparticle. Successful calibration curve for Ca was achieved through human teeth sample deposition by immersion tooth substrate with aluminum oxide NPs dissolved in deionized water and ZnO-eugenol paste re-solidification. It was found that the spectrum emission from the ZnO modified tooth surface exhibited a more significant enhancement compared to the unmodified surface. This method ensures a uniform distribution of nanoparticles over the tooth substrate surface. The NELIBS signal enhancement method can be applied in quantitative analytical applications for human teeth samples, provided that: (i) the substrate fabrication process is highly consistent, (ii) the surface coverage and type of nanoparticles are carefully regulated, and (iii) a uniform deposition of human teeth samples is achieved.