Protection of Arsenic Induced Oxidative Stress in Reproductive Organs and Female Infertility by Pectic Polysaccharide of Momordica charantia: An In Vivo Study in dose Dependent Manner

Arsenic is a serious environmental pollutant and reprotoxic agent. Pectic Polysaccharide (CCPS) from Momordica charantia is an antioxidant used to treat various disorders. This study aimed to elucidate the effect of different and effective doses of CCPS on the female reproductive system against arsenic-induced toxicity by co-administration mode. Thirty rats were equally distributed into five groups. Designated as Control, As^III+ (10.0 mg/Kg Body-Weight) and Co-treatment of CCPS in different doses (1.5 mg/Kg BW), CCPS (2.0 mg/Kg BW), CCPS (2.5 mg/Kg BW). Rats were administered their respective doses orally every day for eight days. Here, the most effective doses at CCPS (2.0 mg/Kg BW) and CCPS (2.5 mg/Kg BW) significantly improved As^III+ induced uterine lipid peroxidation state and ROS generation by the re-establishment of Superoxide Dismutase and catalase activities. In addition, these doses (2.0 mg and 2.5 mg) at CCPS improved ovarian steroidogenesis activities and restored uterine-arsenic-treated rats by controlling LH, FSH, and estradiol levels. The present study revealed that Isolated CCPS obtained the D-galactose and D-methyl galacturonate components (1:4), and the IR spectrum indicated a possible chelating property of CCPS with the presence of binding sites (OH–/COOH) for arsenic. So, We conclude that galacturonate acid residue in doses at CCPS (2.0 mg/Kg BW) and CCPS (2.5 mg/Kg BW) CCPS may contribute a potential interaction with sodium arsenite and play a critical role against arsenic-mediated uterine and ovarian toxicity by the possible regulation of S-adenosine methionine (SAM) pool.