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<p>The study is designed to design a mathematical model to evaluate the synergism of two pairs of extracted phytoconstituents of Bayberry (<i>Myrica esculenta</i>) and Blueberry (<i>Vaccinium corymbosum</i>) on their anticancer effect by Synergy finder 3.0 software. The herbs were collected from the Jim Corbett region of the Himalayan foothill. The active constituents were extracted using Soxhlet with methanol solvent, and further purified. The isolated compounds were tested for total phenol content, antioxidant activity, anticancer activity, cell viability, and cytotoxicity. The study was followed by evaluating the antagonistic or synergistic effect of isolated components using Synergy finder software. The methanolic extracts of Bayberry were found to have the highest TPC of 35.4 (mg/g) and more radical scavenging activity than 28.9(mg/g) of Blueberry. The maximal percentage of cell viability for the methanolic fraction of Blueberry and Bayberry both at 10&#xa0;µg/mL was found to be 97.24 ± 1.10 and 94.38 ± 4.60, respectively. Quercetin from Blueberry; ellagic acid and resveratrol from Bayberry were purified and exhibited anticancer activity. IC50 of quercetin was found to be 4.92&#xa0;µg/ml, while for resveratrol and ellagic acid was 6.97 and 1.285&#xa0;µg/ml, respectively. The computational studies revealed an optimum synergistic effect of the combinations at a concentration of 4&#xa0;µg/ml quercetin, 1.5&#xa0;µg/ml ellagic acid, and 0.5&#xa0;µg/ml resveratrol, which resulted in complete inhibition of HeLa cell viability. The computational research establishes a synergistic anticancer effect of the bioactives isolated from the Blueberry and Bayberry owing to their antioxidant activity and total phenolic content.</p>

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Synergyfinder drug combination screening of bioactives from Myrica esculenta and Vaccinium corymbosum on cervical cell lines

  • 期刊论文

  • 发表日期：

    2025年6月7日

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  • Vipresh Bhardwaj,
  • G. T. Kulkarni,
  • Kalpana Nagpal

摘要

The study is designed to design a mathematical model to evaluate the synergism of two pairs of extracted phytoconstituents of Bayberry (Myrica esculenta) and Blueberry (Vaccinium corymbosum) on their anticancer effect by Synergy finder 3.0 software. The herbs were collected from the Jim Corbett region of the Himalayan foothill. The active constituents were extracted using Soxhlet with methanol solvent, and further purified. The isolated compounds were tested for total phenol content, antioxidant activity, anticancer activity, cell viability, and cytotoxicity. The study was followed by evaluating the antagonistic or synergistic effect of isolated components using Synergy finder software. The methanolic extracts of Bayberry were found to have the highest TPC of 35.4 (mg/g) and more radical scavenging activity than 28.9(mg/g) of Blueberry. The maximal percentage of cell viability for the methanolic fraction of Blueberry and Bayberry both at 10 µg/mL was found to be 97.24 ± 1.10 and 94.38 ± 4.60, respectively. Quercetin from Blueberry; ellagic acid and resveratrol from Bayberry were purified and exhibited anticancer activity. IC50 of quercetin was found to be 4.92 µg/ml, while for resveratrol and ellagic acid was 6.97 and 1.285 µg/ml, respectively. The computational studies revealed an optimum synergistic effect of the combinations at a concentration of 4 µg/ml quercetin, 1.5 µg/ml ellagic acid, and 0.5 µg/ml resveratrol, which resulted in complete inhibition of HeLa cell viability. The computational research establishes a synergistic anticancer effect of the bioactives isolated from the Blueberry and Bayberry owing to their antioxidant activity and total phenolic content.