Scholarlink

<p>In vivo functional imaging is a powerful tool for identifying the functional organization of the cerebral cortex. Flavoprotein autofluorescence imaging detects the metabolic activity of endogenous flavoprotein in neuronal tissue and thus provides an indirect measure of brain activity. As flavoprotein imaging is minimally invasive and does not require expensive equipment or reagents, it is easily combined with tract tracing methods and serves as a valuable approach for investigating functional organization of the brain. This paper provides a step-by-step protocol for flavoprotein imaging to record cortical activity in response to auditory stimuli, along with a method for injecting neuroanatomical tracers into functionally identified cortical regions. Key factors for obtaining reproducible results include stabile head fixation, a controlled dark imaging environment, maintenance of body temperature, and oxygen saturation monitoring.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

In vivo flavoprotein functional imaging of the mouse auditory cortex combined with neuroanatomical tracing

  • 期刊论文

  • 发表日期：

    2025年6月2日

查看全文
  • Tetsufumi Ito,
  • Yosuke Hirayama

摘要

In vivo functional imaging is a powerful tool for identifying the functional organization of the cerebral cortex. Flavoprotein autofluorescence imaging detects the metabolic activity of endogenous flavoprotein in neuronal tissue and thus provides an indirect measure of brain activity. As flavoprotein imaging is minimally invasive and does not require expensive equipment or reagents, it is easily combined with tract tracing methods and serves as a valuable approach for investigating functional organization of the brain. This paper provides a step-by-step protocol for flavoprotein imaging to record cortical activity in response to auditory stimuli, along with a method for injecting neuroanatomical tracers into functionally identified cortical regions. Key factors for obtaining reproducible results include stabile head fixation, a controlled dark imaging environment, maintenance of body temperature, and oxygen saturation monitoring.