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<p>Pelagic fish represent a major proportion of global fisheries, but traditional fisheries survey and monitoring tools are time-consuming. We have compared the results of environmental DNA (eDNA) metabarcoding with those of traditional egg/larval surveys using oblique tow BONGO nets and vertical tow North Pacific Standard (NORPAC) nets for six pelagic fishes in the spawning grounds of the western Japanese waters to examine the effectiveness of eDNA surveys. The detection of 52 species by eDNA was considered to be reasonable based on their natural distribution. The results using the BONGO net, which had the largest filtration volume, indicated a wider presence for six fishes than&#xa0;the results using eDNA metabarcoding and the NORPAC net. eDNA detection results for round herring <i>Etrumeus micropus</i>, Japanese sardine <i>Sardinops melanostictus</i>, and Japanese anchovy <i>Engraulis japonicus</i> were more strongly correlated with observation results for the NORPAC net than those for the BONGO net. In contrast, no correlations were observed between eDNA and two&#xa0;net surveys for Japanese jack mackerel <i>Trachurus japonicus</i> and mackerel (both <i>Scomber japonicus</i> and <i>Scomber australasicus</i>). The results indicate the effectiveness of eDNA surveys for understanding spatial distribution, especially for round herring, sardine, and anchovy during their spawning. As there were several presence results only by eDNA, this method potentially complements traditional surveys.</p>

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Spatial comparison of environmental DNA metabarcoding and traditional egg/larval surveys for pelagic fishes in the spawning grounds of the western Japanese waters

  • 期刊论文

  • 发表日期：

    2025年8月20日

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  • Haruhiko Hino,
  • Kay Sakuma,
  • Satoshi Kitajima,
  • Taketoshi Kodama

摘要

Pelagic fish represent a major proportion of global fisheries, but traditional fisheries survey and monitoring tools are time-consuming. We have compared the results of environmental DNA (eDNA) metabarcoding with those of traditional egg/larval surveys using oblique tow BONGO nets and vertical tow North Pacific Standard (NORPAC) nets for six pelagic fishes in the spawning grounds of the western Japanese waters to examine the effectiveness of eDNA surveys. The detection of 52 species by eDNA was considered to be reasonable based on their natural distribution. The results using the BONGO net, which had the largest filtration volume, indicated a wider presence for six fishes than the results using eDNA metabarcoding and the NORPAC net. eDNA detection results for round herring Etrumeus micropus, Japanese sardine Sardinops melanostictus, and Japanese anchovy Engraulis japonicus were more strongly correlated with observation results for the NORPAC net than those for the BONGO net. In contrast, no correlations were observed between eDNA and two net surveys for Japanese jack mackerel Trachurus japonicus and mackerel (both Scomber japonicus and Scomber australasicus). The results indicate the effectiveness of eDNA surveys for understanding spatial distribution, especially for round herring, sardine, and anchovy during their spawning. As there were several presence results only by eDNA, this method potentially complements traditional surveys.