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<p>Cosavirus (CoSV) and Salivirus (SalV) are two novel picornaviruses that are associated with human diseases. However, epidemiological data on these viruses in China remain limited. In this study, we conducted the first simultaneous investigation of CoSV and SalV in a city of eastern China by wastewater-based epidemiology (WBE) method. From 2021 to 2023, a total of 21 influent wastewater samples were collected. After virus concentration and molecular detection, 71.43% (15/21) and 80.95% (17/21) of samples tested positive for CoSV and SalV, respectively. The sequences of CoSV were determined to be CoSV-A and CoSV-D, and SalV were all genotyped to be SalV-A1. Phylogenetic analysis showed that UTR region of CoSV-D and 3D region of CoSV-A can be divided into several clusters. UTR region of CoSV-A, UTR and 3D region of SalV-A1 were not clustered. Furthermore, the sequences in this study were closely related to some strains from human samples in China and wastewater samples in other countries. These findings reveal that CoSV and SalV circulated at a high prevalence in the local population and demonstrate that WBE is an effective method for novel picornaviruses research.</p>

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Detection and Genetic Characteristics of Cosavirus and Salivirus in the Wastewater, China

  • 期刊论文

  • 发表日期：

    2025年8月15日

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  • Nan Zhou,
  • Yue Huang,
  • Tong Shen,
  • Tingting Xiao,
  • Hanyue Pang,
  • Xinyu Shen

摘要

Cosavirus (CoSV) and Salivirus (SalV) are two novel picornaviruses that are associated with human diseases. However, epidemiological data on these viruses in China remain limited. In this study, we conducted the first simultaneous investigation of CoSV and SalV in a city of eastern China by wastewater-based epidemiology (WBE) method. From 2021 to 2023, a total of 21 influent wastewater samples were collected. After virus concentration and molecular detection, 71.43% (15/21) and 80.95% (17/21) of samples tested positive for CoSV and SalV, respectively. The sequences of CoSV were determined to be CoSV-A and CoSV-D, and SalV were all genotyped to be SalV-A1. Phylogenetic analysis showed that UTR region of CoSV-D and 3D region of CoSV-A can be divided into several clusters. UTR region of CoSV-A, UTR and 3D region of SalV-A1 were not clustered. Furthermore, the sequences in this study were closely related to some strains from human samples in China and wastewater samples in other countries. These findings reveal that CoSV and SalV circulated at a high prevalence in the local population and demonstrate that WBE is an effective method for novel picornaviruses research.