<p>Calcium entry through voltage-gated calcium channels as well as calcium release from intracellular calcium depots is crucial for triggering the release of neurotransmitters, synaptic plasticity and regulating the activity of various intracellular proteins. Recent evidence suggests that neuronal calcium signaling is impaired in many neurodegenerative disorders. This review presents methodological approaches that allow the estimation of the dynamics of calcium entry in motor nerve endings using fluorescent calcium indicators. The methods considered were: selecting calcium dyes depending on the experimental conditions, and loading indicators into the cells of interest. This review outlines general principles and techniques to record fast fluorescent calcium signals. Possible results that can be obtained within the framework of experiments using the described methods are also presented.</p>