<p>The consumption of milk can contribute to the consumers` exposure to aflatoxin as aflatoxin M₁ (AFM₁), a hydroxylated metabolite of aflatoxin B₁ (AFB₁), is transferred into the milk of AFB₁ exposed dairy cows. As mitigation measures against AFB₁ presence in cattle feed, fungal biocontrol strains are applied on plant materials to reduce toxigenic <i>A. flavus</i> growth. The present study investigates the impact of applying two biocontrol strains (<i>Trichoderma afroharzianum</i> and non-toxigenic <i>Aspergillus flavus</i>) compared with a toxigenic <i>A. flavus</i> strain on the mycotoxin transfer from maize into milk and milk products in a “farm to fork” approach. Firstly, in a feeding study three groups of high yielding dairy cows are exposed to different maize flours infested with biocontrol or AFB₁-foming fungal strains for 14 days to monitor a potential toxin transfer from feed to milk. In the milk of cows exposed to AFB₁, AFM₁ was detected with a mean value of (95.6 ng/kg ± 13.7 ng/kg) while the mean transfer rates (3.8% ± 0.9%) were slightly higher than published in previous studies. Neither AFM₁ nor cyclopiazonic acid could be detected in milk from the cows exposed to the two biocontrol strains. The data show, that the AFM₁ limit of 50 ng/kg in milk can be exceeded when cattle feed is contaminated with AFB₁ within the legal limit. Consequently, the use of cattle feed within the current European legal limits does not necessarily guarantee for obtaining milk compliant with European maximum levels. In the second part, AFM₁ containing raw milk from the experimental cows was processed further into yoghurt to investigate the fate of the toxin under acidic fermentation conditions. No AFM₁ degradation was found during authentic yoghurt making, albeit in a model experiment the hydration of AFM₁ in presence of citric acid was shown. Further studies are necessary to investigate the exact conditions suitable for reducing the AFM₁ content of contaminated milk during the processing of dairy products</p>