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<p>An integrated analysis for the identification of spiny lobster species (<i>Panulirus</i> spp.) from the waters of Southern Java and Buton Island was conducted by combining morphological, morphometric, and molecular data. Molecular data, based on the mitochondrial 16S rRNA gene, provided DNA sequences ranging from 436 to 496&#xa0;bp. Together with morphological evidence, this data consistently identified five distinct species: <i> P.&#xa0;homarus</i>, <i>P. versicolor</i>, <i>P. longipes</i>, <i>P. penicillatus</i>, and <i>P. ornatus</i>. Although morphometric analysis suggests the posterior carapace as a potential distinguishing character, Principal Component Analysis (PCA) plots show significant overlap, confirming the high morphological similarity among conspecifics and the potential risk of misidentification. In contrast, molecular data definitively resolved the taxonomic status of all species, as evidenced by high sequence identity (&gt; 97%), clear barcoding gaps between intraspecific (0–1%) and interspecific (4–22%) genetic distances, and robustly supported phylogenetic trees. The findings of our study emphasize the importance of using molecular markers to complement morphological approaches. This integrated framework provides a solid foundation for accurate species determination, which is essential for the effective management and conservation of <i>Panulirus</i> resources.</p>

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X′ morphology, morphometry, and molecular identification of lobsters (Panulirus spp.) in the Southern Java Sea and Buton Island

  • 期刊论文

  • 发表日期：

    2026年5月11日

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  • Agita Ashya Azani,
  • Ratih Ida Adharini,
  • Riza Yuliratno Setiawan,
  • Eko Hardianto,
  • Muhamad Rijal

摘要

An integrated analysis for the identification of spiny lobster species (Panulirus spp.) from the waters of Southern Java and Buton Island was conducted by combining morphological, morphometric, and molecular data. Molecular data, based on the mitochondrial 16S rRNA gene, provided DNA sequences ranging from 436 to 496 bp. Together with morphological evidence, this data consistently identified five distinct species: P. homarus, P. versicolor, P. longipes, P. penicillatus, and P. ornatus. Although morphometric analysis suggests the posterior carapace as a potential distinguishing character, Principal Component Analysis (PCA) plots show significant overlap, confirming the high morphological similarity among conspecifics and the potential risk of misidentification. In contrast, molecular data definitively resolved the taxonomic status of all species, as evidenced by high sequence identity (> 97%), clear barcoding gaps between intraspecific (0–1%) and interspecific (4–22%) genetic distances, and robustly supported phylogenetic trees. The findings of our study emphasize the importance of using molecular markers to complement morphological approaches. This integrated framework provides a solid foundation for accurate species determination, which is essential for the effective management and conservation of Panulirus resources.