<p>Our previous study of the sugar-regulated expression of <i>OsHXK7</i> uncovered a functional link between sugar and methyl jasmonate (MJ) signaling in rice. Further examination of additional sugar-responsive genes, including <i>Amy3D</i> and <i>CIPK15</i>, indicated that MJ interferes with sugar-mediated repression. To access global transcriptomic overlap, we performed RNA-seq analysis on rice embryos treated with or without glucose and MJ. About 70% of sugar-responsive genes were also MJ-responsive. Notably, MJ generally enhanced the expression of sugar-repressed genes while preferentially attenuating the expression of sugar-induced genes, revealing an overall antagonistic relationship between the two pathways. Under sugar-deficient conditions, the number of MJ-responsive genes increased about fivefold compared with sugar-rich conditions, and the magnitude of responsiveness to MJ was also conversely affected by sugar conditions, suggesting that MJ responsiveness is strongly influenced by sugar availability. Among the C and S₁ subfamilies of OsbZIP transcription factors—previously implicated in maintaining energy homeostasis—we identified several members capable of transactivating the <i>OsHXK7</i> and <i>Amy3D</i> promoters. All C/S₁ OsbZIP genes were induced by MJ, and many of them were also responsive to sugar, suggesting a possible regulatory network that fine-tunes gene expression in response to combined metabolic and hormonal cues.</p>