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<p><i>Rhododendron</i>play a crucial role in landscaping and as indoor ornamental plants due to their diverse species and rich colors. However, the molecular mechanisms governing the formation of these colors remain largely unknown. In our study, we utilized RNA-seq and identified that the genes <i>RhCHI</i>, <i>RhF3H</i>, and <i>RhANS</i>, are pivotal for anthocyanin biosynthesis, directing anthocyanin accumulation. qRT-PCR analyses revealed the expression levels of these differentially expressed genes (DEGs) in anthocyanin synthesis. Subsequently, in vitro experiments confirmed the up-regulation of encoded proteins. In <i>Arabidopsis thaliana</i>, plants induced with anthocyanin demonstrated significantly higher expression levels compared to the wild-type. These findings demonstrate that <i>RhCHI</i>, <i>RhF3H</i>, and <i>RhANS</i> regulate anthocyanin synthesis, laying the groundwork for further exploration of <i>Rhododendron</i>. This study provides critical genetic targets and methodologies for molecular breeding of <i>Rhododendron</i> cultivars with enhanced flower coloration and stress resilience, advancing both ornamental horticulture and plant synthetic biology.</p>

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Transcriptome Analysis and Functional Verification of Key Genes Involved in Anthocyanin Biosynthesis in Rhododendron

  • 期刊论文

  • 发表日期：

    2025年8月29日

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  • Chenfei Zhang,
  • Qinghao Wang,
  • Baoxin Jiang,
  • Yonghong Jia,
  • Huixia Shou,
  • Zhihui Chen,
  • Yuhao Cheng,
  • Yueyan Wu

摘要

Rhododendronplay a crucial role in landscaping and as indoor ornamental plants due to their diverse species and rich colors. However, the molecular mechanisms governing the formation of these colors remain largely unknown. In our study, we utilized RNA-seq and identified that the genes RhCHI, RhF3H, and RhANS, are pivotal for anthocyanin biosynthesis, directing anthocyanin accumulation. qRT-PCR analyses revealed the expression levels of these differentially expressed genes (DEGs) in anthocyanin synthesis. Subsequently, in vitro experiments confirmed the up-regulation of encoded proteins. In Arabidopsis thaliana, plants induced with anthocyanin demonstrated significantly higher expression levels compared to the wild-type. These findings demonstrate that RhCHI, RhF3H, and RhANS regulate anthocyanin synthesis, laying the groundwork for further exploration of Rhododendron. This study provides critical genetic targets and methodologies for molecular breeding of Rhododendron cultivars with enhanced flower coloration and stress resilience, advancing both ornamental horticulture and plant synthetic biology.