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<p>Millet, the main small miscellaneous grain in our country, and it is also one of the model crops. Drought is one of the main factor leading to the severe yield reduction of millet crops, and the study of drought tolerance genes in millet can provide new ideas for improving yield. <i>ORANGE </i>(<i>OR</i>) is a gene first discovered in orange cauliflower mutants, which can enhance plant adaptation to environmental stress, but <i>ORANGE</i> gene in millet has not been studied. In this study, in order to analyze the gene in plant drought stress, we analyzed the expression pattern of <i>SiOR</i> gene in various tissues of the millet by qPCR, and the spatiotemporal expression pattern of <i>SiOR</i> gene in millet ear under natural drought. We cloned and isolated the <i>SiOR</i> gene to obtain <i>SiOR</i>-overexpressing transgenic <i>Arabidopsis </i>and further studied the growth in different periods under drought stress and determine indicators related to drought resistance at seedling stage. We found that compared with wild-type WT, the germination rate and seedling growth of overexpressing <i>SiOR Arabidopsis</i> were significantly different. <i>SiOR Arabidopsis</i> was more tolerant than WT under mannitol-mimicking drought stress. Under natural drought conditions at the seedling stage, the survival rate of WT was significantly lower than that of <i>SiOR</i> transgenic <i>Arabidopsis</i>, WT almost wilted or even died, while <i>SiOR</i> transgenic <i>Arabidopsis</i> leaves turned yellow. The content of proline and chlorophyll were significantly higher than those of WT, and the contents of malondialdehyde and hydrogen peroxide were significantly lower than those of WT, indicating that <i>SiOR</i> gene can improve the antioxidant capacity and help enhance the drought tolerance of plants.</p>

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Overexpression of SiOR Enhances Drought Tolerance by Strengthening Antioxidant Defense and Stomatal Closure via ABA Signaling in Arabidopsis thaliana

  • 期刊论文

  • 发表日期：

    2025年7月8日

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  • Minjing Kong,
  • Jingjing Cao,
  • Xiaoqian Fan,
  • Xin Yang,
  • Yajing Sun,
  • Guiqiang Yang,
  • Li Li,
  • Meiping Zhang

摘要

Millet, the main small miscellaneous grain in our country, and it is also one of the model crops. Drought is one of the main factor leading to the severe yield reduction of millet crops, and the study of drought tolerance genes in millet can provide new ideas for improving yield. ORANGE (OR) is a gene first discovered in orange cauliflower mutants, which can enhance plant adaptation to environmental stress, but ORANGE gene in millet has not been studied. In this study, in order to analyze the gene in plant drought stress, we analyzed the expression pattern of SiOR gene in various tissues of the millet by qPCR, and the spatiotemporal expression pattern of SiOR gene in millet ear under natural drought. We cloned and isolated the SiOR gene to obtain SiOR-overexpressing transgenic Arabidopsis and further studied the growth in different periods under drought stress and determine indicators related to drought resistance at seedling stage. We found that compared with wild-type WT, the germination rate and seedling growth of overexpressing SiOR Arabidopsis were significantly different. SiOR Arabidopsis was more tolerant than WT under mannitol-mimicking drought stress. Under natural drought conditions at the seedling stage, the survival rate of WT was significantly lower than that of SiOR transgenic Arabidopsis, WT almost wilted or even died, while SiOR transgenic Arabidopsis leaves turned yellow. The content of proline and chlorophyll were significantly higher than those of WT, and the contents of malondialdehyde and hydrogen peroxide were significantly lower than those of WT, indicating that SiOR gene can improve the antioxidant capacity and help enhance the drought tolerance of plants.