<p>Enhancers are indispensable <i>cis</i>-regulatory elements that fine-tune gene expression, orchestrating complex developmental and environmental responses in living systems. Although the importance of enhancers is well established in animal systems, less is known about enhancers in plants, largely due to the lack of typical features defining these elements. Recent breakthroughs in high-throughput reporter assays have shed light on plant enhancers and their unique genetic features. In this review, we introduce a range of methodologies currently used to study plant enhancers, including large-scale analysis of chromatin accessibility, high-throughput reporter assays, and chromatin conformation capture techniques. Accumulating evidence supports the notion that the activities of plant enhancers are strongly dependent on their genomic positions, unlike the position-independent enhancers described in animal systems. We also summarize the experimentally validated functions of plant enhancers, underscoring how enhancers help fine-tune gene expression and various biological processes.</p>