<p>Throughout history the Zagreb Cathedral was built and rebuilt several times, mostly by using limestone types from quarries located at the margins of the Medvednica Mt. (Northern Croatia). Any restoration of a valuable historic building requires an investigation of the materials from which it was built, especially because the usage of local materials contributes to the promotion of local geoheritage through economic, cultural, aesthetic and educational values. This study focuses on the analysis of limestone types used in the restoration of the Zagreb Cathedral, including lithothamnium limestone and sandstone dislodged from the Cathedral, lithothamnium limestone and litavac limestone from the Bizek quarry as well as travertine used for its renovation. In contrast to most geological investigations, in which fresh, undamaged rock samples are examined, these investigations include samples that have been embedded in the building for a long time. The basic properties of various natural stone samples were investigated, including mineral composition by X-ray diffraction, water absorption under atmospheric pressure, apparent density, open porosity, sodium sulfate durability and resistance to frost, salt crystallization and acid rain. Our results suggest that lithothamnium limestone, litavac limestone and sandstone are not suitable building materials due to their high water absorption and sensitivity to weathering and durability during tests, especially compared to travertine. These types of investigations are valuable because they provide information whether the original construction materials of historic buildings are suitable for the long-term preservation of buildings during restoration.</p>