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<p>There is a transition in production of probiotics from milk-based sources to plant-based ones. Even though sucrose-based fermentation offers benefits, the cost of purified sucrose hampers the economics of probiotic production. Sugarcane juice (SJ) appears as a cost-effective and renewable feedstock, for such applications; however, its use is restricted owing to huge loads of antimicrobial phenolics. Removal of phenolics from SJ by reverse micellar extraction, makes it a cost-effective feedstock for fermentative production of <i>Lactobacillus plantarum</i> NCIM2083. The physical parameters of fermentation were individually optimized. Media composition was optimized using a two-level experimental design strategy. Plackett–Burman screening design was applied to identify media components having a significant effect on biomass production and their levels were optimized by a rotatable central composite design to achieve a viable cell count of 10.028 ± 0.375 log CFU/mL. These optimizations improved biomass accumulation by ~ 1.7 times. Kinetic modelling of batch fermentation was also attempted for evaluation of the process. Usability of SJ as a suitable feedstock for production of probiotics was successfully demonstrated. This study demonstrates the feasibility of using clarified sugarcane juice in microbial fermentations and opens new horizons for sucrose-based microbial fermentation processes using minimally processed sugarcane juice.</p>

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Sugarcane Juice was Strategically Evaluated as a Feedstock for Sustainable Manufacturing of Bioproducts: Probiotics

  • 期刊论文

  • 发表日期：

    2026年5月6日

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  • Krushna Gharat,
  • Harsha G. Madhankar,
  • Kshitija Japhalekar,
  • Kashyap Dharini,
  • Rekha S. Singhal

摘要

There is a transition in production of probiotics from milk-based sources to plant-based ones. Even though sucrose-based fermentation offers benefits, the cost of purified sucrose hampers the economics of probiotic production. Sugarcane juice (SJ) appears as a cost-effective and renewable feedstock, for such applications; however, its use is restricted owing to huge loads of antimicrobial phenolics. Removal of phenolics from SJ by reverse micellar extraction, makes it a cost-effective feedstock for fermentative production of Lactobacillus plantarum NCIM2083. The physical parameters of fermentation were individually optimized. Media composition was optimized using a two-level experimental design strategy. Plackett–Burman screening design was applied to identify media components having a significant effect on biomass production and their levels were optimized by a rotatable central composite design to achieve a viable cell count of 10.028 ± 0.375 log CFU/mL. These optimizations improved biomass accumulation by ~ 1.7 times. Kinetic modelling of batch fermentation was also attempted for evaluation of the process. Usability of SJ as a suitable feedstock for production of probiotics was successfully demonstrated. This study demonstrates the feasibility of using clarified sugarcane juice in microbial fermentations and opens new horizons for sucrose-based microbial fermentation processes using minimally processed sugarcane juice.