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<p>Xylanases are enzymes that convert xylan into simple sugars, serving as additional substrates for enhancing the production of bioethanol and other value-added products, offering a potential approach for sustainable biomass conversion. Our research focuses on a cost-effective and environmentally friendly approach. Therefore, a recombinant microbe expressing endo-1,4-<i>β</i>-xylanase (<i>xynA</i>) was developed and utilised for the hydrolysis of corn cob xylan, lab-extracted sugarcane bagasse xylan, and untreated sugarcane bagasse to increase the production of fermentable sugars. This study reports the use of whole-cell biomass for the first time, targeting the hydrolysis of xylan in raw biomass. Moreover, the ability of the cloned bacterium to overproduce its donor was assessed through a comparative depolymerization. The recombinant <i>E. coli</i> has shown 18–62% more xylose production during 24–60h of induction and 54.68% higher production of xylose after 24h of incubation, in commercial xylan and raw biomass, respectively, compared to <i>B. subtilis</i>. The results demonstrated an efficient alternative to the enzyme purification process, providing a simpler method for valorising the lignocellulosic biomass in a biorefinery.</p>

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Sustainable Valorisation of Untreated Sugarcane Bagasse and its Extracted Xylan: A Comparative Study of Enhanced Xylanolytic Potential among Engineered E. Coli and Donor Bacterium

  • 期刊论文

  • 发表日期：

    2026年5月6日

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  • Ayush Saxena,
  • Fouziya Parveen,
  • Akhtar Hussain,
  • Mohd Khubaib,
  • Mohammad Ashfaque

摘要

Xylanases are enzymes that convert xylan into simple sugars, serving as additional substrates for enhancing the production of bioethanol and other value-added products, offering a potential approach for sustainable biomass conversion. Our research focuses on a cost-effective and environmentally friendly approach. Therefore, a recombinant microbe expressing endo-1,4-β-xylanase (xynA) was developed and utilised for the hydrolysis of corn cob xylan, lab-extracted sugarcane bagasse xylan, and untreated sugarcane bagasse to increase the production of fermentable sugars. This study reports the use of whole-cell biomass for the first time, targeting the hydrolysis of xylan in raw biomass. Moreover, the ability of the cloned bacterium to overproduce its donor was assessed through a comparative depolymerization. The recombinant E. coli has shown 18–62% more xylose production during 24–60h of induction and 54.68% higher production of xylose after 24h of incubation, in commercial xylan and raw biomass, respectively, compared to B. subtilis. The results demonstrated an efficient alternative to the enzyme purification process, providing a simpler method for valorising the lignocellulosic biomass in a biorefinery.