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<p>Enzymatic catalysis has made significant breakthroughs in the decolorization of molasses wastewater. However, challenges such as the difficulty in recovering free enzymes hinder the application of this technology on an industrial scale and limit its development. This study introduces a low deep eutectic solvent-assisted immobilization technique (DES-IM) to explore the potential application of lipase in the decolorization of molasses wastewater. The results have demonstrated that immobilized lipase (Lecitase® Ultra) retained 53.29% of its initial activity after 6 h of continuous operation at elevated temperatures. Furthermore, under strong acidic and alkaline conditions, it maintained residual activities of 68.17% and 72.30%, respectively; this activity under extreme conditions significantly exceeded that of IM-Ultra. Additionally, experiments conducted with industrial-grade molasses wastewater indicated that DES-IM-Ultra improved decolorization efficiency by 12.03% compared to IM-Ultra, achieving an impressive decolorization efficiency of 87.48%. In conclusion, this technology demonstrates strong reproducibility, high stability, and effective decolorization. It offered a more environmentally friendly and efficient strategy for the industrial decolorization of molasses wastewater.</p>

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Development and Application of a Method to Enhance Lipase Performance and Wastewater Treatment Capacity Using DES

  • 期刊论文

  • 发表日期：

    2026年2月23日

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  • Jiaxin Song,
  • Chuanlong Yu,
  • Fangling Wu,
  • Li Yuan,
  • Danna Fan,
  • Yiheng Zhang,
  • Yuping You,
  • Guoqiang Wu,
  • Zedong Zhang,
  • Wenjun Wang

摘要

Enzymatic catalysis has made significant breakthroughs in the decolorization of molasses wastewater. However, challenges such as the difficulty in recovering free enzymes hinder the application of this technology on an industrial scale and limit its development. This study introduces a low deep eutectic solvent-assisted immobilization technique (DES-IM) to explore the potential application of lipase in the decolorization of molasses wastewater. The results have demonstrated that immobilized lipase (Lecitase® Ultra) retained 53.29% of its initial activity after 6 h of continuous operation at elevated temperatures. Furthermore, under strong acidic and alkaline conditions, it maintained residual activities of 68.17% and 72.30%, respectively; this activity under extreme conditions significantly exceeded that of IM-Ultra. Additionally, experiments conducted with industrial-grade molasses wastewater indicated that DES-IM-Ultra improved decolorization efficiency by 12.03% compared to IM-Ultra, achieving an impressive decolorization efficiency of 87.48%. In conclusion, this technology demonstrates strong reproducibility, high stability, and effective decolorization. It offered a more environmentally friendly and efficient strategy for the industrial decolorization of molasses wastewater.