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<p>Post-harvest degradation of sugarcane, mainly based on formation of dextran, significantly affects sucrose recovery, a critical concern for sugar-producing nations. Dextranase, an enzyme capable of hydrolyzing dextran presents a potential solution to mitigate this issue. The current study investigates the ultrasound (US)-assisted intensification of dextranase activity under varying parameters, including ultrasonic frequency, power, duty cycle, and system parameters such as temperature and enzyme concentration. Ultrasonic bath and horn were employed for dextran hydrolysis to understand the effect of type of ultrasonic reactor. Initial dextranase activity was measured at 281.2&#xa0;U/g under optimal conditions of 50&#xa0;°C and pH 5. Following 15-min sonication in the ultrasonic bath, maximum enzyme activity reached 444.8&#xa0;U/g at 60&#xa0;W power and 20% duty cycle, elucidated as optimum conditions. In contrast, the ultrasonic horn reactor yielded a lower maximum activity of 388.6&#xa0;U/g under conditions of power as 40&#xa0;W, a 40% duty cycle, and the same exposure time. The intensified dextranase was subsequently applied to sugarcane juice processing, demonstrating improved hydrolysis of dextran and highlighting the potential benefits of US-assisted enzymatic treatments in industrial applications. The findings provide a foundation for optimizing enzymatic processes in sugar production and addressing post-harvest sugarcane degradation.</p>

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Ultrasound-Assisted Intensification of Dextranase Activity and Subsequent Hydrolysis of Dextran Present in Sugarcane Juice

  • 期刊论文

  • 发表日期：

    2026年2月12日

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  • G. Nishchitha,
  • Priya Yadav,
  • Parag R. Gogate

摘要

Post-harvest degradation of sugarcane, mainly based on formation of dextran, significantly affects sucrose recovery, a critical concern for sugar-producing nations. Dextranase, an enzyme capable of hydrolyzing dextran presents a potential solution to mitigate this issue. The current study investigates the ultrasound (US)-assisted intensification of dextranase activity under varying parameters, including ultrasonic frequency, power, duty cycle, and system parameters such as temperature and enzyme concentration. Ultrasonic bath and horn were employed for dextran hydrolysis to understand the effect of type of ultrasonic reactor. Initial dextranase activity was measured at 281.2 U/g under optimal conditions of 50 °C and pH 5. Following 15-min sonication in the ultrasonic bath, maximum enzyme activity reached 444.8 U/g at 60 W power and 20% duty cycle, elucidated as optimum conditions. In contrast, the ultrasonic horn reactor yielded a lower maximum activity of 388.6 U/g under conditions of power as 40 W, a 40% duty cycle, and the same exposure time. The intensified dextranase was subsequently applied to sugarcane juice processing, demonstrating improved hydrolysis of dextran and highlighting the potential benefits of US-assisted enzymatic treatments in industrial applications. The findings provide a foundation for optimizing enzymatic processes in sugar production and addressing post-harvest sugarcane degradation.