Scholarlink

<p>Cellulases and xylanases are enzymes whose demand has grown in recent years; they have various applications, including biofuels production from lignocellulosic materials, also called second-generation biofuels (2G). <i>Trichoderma</i> is a fungus that produces cellulases and xylanases enzymes. In this work, the cellulases and xylanases production were optimized by submerged fermentation using sugarcane bagasse and <i>T. harzianum</i> ITV-22 (strain isolated from sugarcane bagasse), using the response surface methodology and the desirability criterion, evaluating the effect of the sugarcane bagasse concentration, nitrogen sources (peptone and ammonium sulfate) and as response variables the cellulase (exoglucanase: FPase, endoglucanase: CMCase), <i>β</i>-glucosidase (BGL)) and xylanase (Xyl) activity, respectively. Through multi-response optimization and desirability function, the best conditions were obtained to simultaneously maximize cellulase and xylanase activity in this work. The desirability function with a <i>d</i> = 0.93 showed the optimal conditions reaching an FPase, CMCase, BGL and Xyl activity of 396.58, 833.03, 11,930.60, 72,393.60&#xa0;U/L, respectively. The experimental results were similar to those predicted by the optimization model with a correlation coefficient greater than 92%. The indigenous strain <i>T. harzianum</i> ITV-22 is capable to produce an enzymatic extract with cellulase and xylanase activity with a 60% conversion of cellulose to glucose in sugarcane bagasse hydrolyses; these results could be favor the second-generation (2G) bioethanol production process.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Optimization of Cellulases and Xylanase Production Using Trichoderma harzianum ITV-22 Isolated from Sugarcane Bagasse

  • 期刊论文

  • 发表日期：

    2025年12月5日

查看全文
  • María Ines Infanzón-Rodríguez,
  • Javier Gómez-Rodríguez,
  • Daniel Arturo Zavala-Ortiz,
  • Leticia López-Zamora,
  • Claudia Castro-Martínez,
  • María Guadalupe Aguilar-Uscanga

摘要

Cellulases and xylanases are enzymes whose demand has grown in recent years; they have various applications, including biofuels production from lignocellulosic materials, also called second-generation biofuels (2G). Trichoderma is a fungus that produces cellulases and xylanases enzymes. In this work, the cellulases and xylanases production were optimized by submerged fermentation using sugarcane bagasse and T. harzianum ITV-22 (strain isolated from sugarcane bagasse), using the response surface methodology and the desirability criterion, evaluating the effect of the sugarcane bagasse concentration, nitrogen sources (peptone and ammonium sulfate) and as response variables the cellulase (exoglucanase: FPase, endoglucanase: CMCase), β-glucosidase (BGL)) and xylanase (Xyl) activity, respectively. Through multi-response optimization and desirability function, the best conditions were obtained to simultaneously maximize cellulase and xylanase activity in this work. The desirability function with a d = 0.93 showed the optimal conditions reaching an FPase, CMCase, BGL and Xyl activity of 396.58, 833.03, 11,930.60, 72,393.60 U/L, respectively. The experimental results were similar to those predicted by the optimization model with a correlation coefficient greater than 92%. The indigenous strain T. harzianum ITV-22 is capable to produce an enzymatic extract with cellulase and xylanase activity with a 60% conversion of cellulose to glucose in sugarcane bagasse hydrolyses; these results could be favor the second-generation (2G) bioethanol production process.