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<p>The extensive application of Ametryn (AMT), a triazine herbicide, in agriculture presents considerable environmental concerns because of its prolonged persistence in soil and water. This study investigates microbial degradation as a potential bioremediation strategy to reduce the environmental impact of AMT in sugarcane fields. We aimed to isolate and characterize AMT-degrading bacteria, determine their minimum inhibitory concentration against AMT, and analyze their growth dynamics in the presence of AMT and nutrient substrates like glucose and ammonium nitrate. The bacterial isolates, mainly <i>Pseudomonas aeruginosa</i> and <i>Ensifer adhaerens</i>, were identified from AMT-contaminated soils in Khuzestan Province, Iran, using 16S rRNA sequencing and biochemical assays. Both species exhibited AMT degradation capability, with <i>P. aeruginosa</i> having an MIC of 150&#xa0;µg/mL and <i>E. adhaerens</i> showing an MIC of 185&#xa0;µg/mL. Spectrophotometric analysis revealed substantial bacterial proliferation in the presence of AMT, with <i>P. aeruginosa</i> displaying a higher growth rate. Additionally, <i>E. adhaerens</i> exhibited improved growth in ammonium nitrate, emphasizing its metabolic adaptability. These findings suggest that these bacterial strains have promising potential for bioremediation applications aimed at reducing AMT contamination under controlled laboratory conditions.</p>

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Pseudomonas aeruginosa and Ensifer adhaerens Degrading Ametryn Herbicide in Sugarcane Fields

  • 期刊论文

  • 发表日期：

    2025年7月3日

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  • Sedigheh Mirzavand,
  • Shiva Dowlatkhah,
  • Milad Aeini,
  • Hossein Moazen Rezamahalleh

摘要

The extensive application of Ametryn (AMT), a triazine herbicide, in agriculture presents considerable environmental concerns because of its prolonged persistence in soil and water. This study investigates microbial degradation as a potential bioremediation strategy to reduce the environmental impact of AMT in sugarcane fields. We aimed to isolate and characterize AMT-degrading bacteria, determine their minimum inhibitory concentration against AMT, and analyze their growth dynamics in the presence of AMT and nutrient substrates like glucose and ammonium nitrate. The bacterial isolates, mainly Pseudomonas aeruginosa and Ensifer adhaerens, were identified from AMT-contaminated soils in Khuzestan Province, Iran, using 16S rRNA sequencing and biochemical assays. Both species exhibited AMT degradation capability, with P. aeruginosa having an MIC of 150 µg/mL and E. adhaerens showing an MIC of 185 µg/mL. Spectrophotometric analysis revealed substantial bacterial proliferation in the presence of AMT, with P. aeruginosa displaying a higher growth rate. Additionally, E. adhaerens exhibited improved growth in ammonium nitrate, emphasizing its metabolic adaptability. These findings suggest that these bacterial strains have promising potential for bioremediation applications aimed at reducing AMT contamination under controlled laboratory conditions.