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<p>In the global sugar beet seed industry, high-yield, high-sugar and disease-resistant three-way hybrids play a pivotal role. Understanding the genetic diversity and genetic distance of paternal polyembryonic pollination lines is crucial for constructing three-way cross-combinations. This study utilized two promoter-targeted molecular markers, CAAT box-derived polymorphism (CBDP) and cis-element amplification polymorphism (CEAP), to analyze genetic diversity and population structure among 72 polyembryonic sugar beet germplasm resources, while establishing their DNA fingerprints. Using TD-PCR, we selected 12 core primers with high polymorphism from CBDP and CEAP markers for genomic DNA amplification. The results showed that a total of 223 alleles were amplified by primers, and the number of alleles was between 4 and 16. Each primer amplified 9 alleles on average. The effective number of alleles (Ne) ranged from 1.0663 to 1.4916, the Shannon information index (<i>I</i>) ranged from 0.0981 to 0.4662, and the expected heterozygosity of Nei ranged from 0.0539 to 0.3037. The polymorphism information content (PIC) of primers ranged from 0.2917 to 0.9583. Population structure analysis conducted using Structure software revealed an optimal <i>K</i> value of 2, indicating that the test materials were segregated into two distinct genetic groups. This grouping pattern showed complete concordance with cluster analysis results. Genetic distances between germplasm accessions ranged from 0.049 to 0.385, with a mean genetic distance of 0.1986. Finally, after screening, all 72 germplasm resources were distinguished by using five primers (CAAT2, TGAC25, GATAA6, AAAG25 and ACGTG4), and the DNA fingerprints of these resources were established.</p>

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Genetic Diversity Analysis of Sugar Beet Polyembryonic Germplasm Resources Based on CBDP and CEAP Molecular Markers

  • 期刊论文

  • 发表日期：

    2025年7月3日

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  • Yue Song,
  • Shengnan Li,
  • Zhi Pi,
  • Zedong Wu

摘要

In the global sugar beet seed industry, high-yield, high-sugar and disease-resistant three-way hybrids play a pivotal role. Understanding the genetic diversity and genetic distance of paternal polyembryonic pollination lines is crucial for constructing three-way cross-combinations. This study utilized two promoter-targeted molecular markers, CAAT box-derived polymorphism (CBDP) and cis-element amplification polymorphism (CEAP), to analyze genetic diversity and population structure among 72 polyembryonic sugar beet germplasm resources, while establishing their DNA fingerprints. Using TD-PCR, we selected 12 core primers with high polymorphism from CBDP and CEAP markers for genomic DNA amplification. The results showed that a total of 223 alleles were amplified by primers, and the number of alleles was between 4 and 16. Each primer amplified 9 alleles on average. The effective number of alleles (Ne) ranged from 1.0663 to 1.4916, the Shannon information index (I) ranged from 0.0981 to 0.4662, and the expected heterozygosity of Nei ranged from 0.0539 to 0.3037. The polymorphism information content (PIC) of primers ranged from 0.2917 to 0.9583. Population structure analysis conducted using Structure software revealed an optimal K value of 2, indicating that the test materials were segregated into two distinct genetic groups. This grouping pattern showed complete concordance with cluster analysis results. Genetic distances between germplasm accessions ranged from 0.049 to 0.385, with a mean genetic distance of 0.1986. Finally, after screening, all 72 germplasm resources were distinguished by using five primers (CAAT2, TGAC25, GATAA6, AAAG25 and ACGTG4), and the DNA fingerprints of these resources were established.