<p>Histological images convey more than just color and shape; they require a layered interpretive process to reveal their full meaning. This contribution argues that histological literacy rests on 3 intertwined modes of interpretation, following semiotic theory. First, the iconic mode helps learners build mental libraries by recognizing visual similarities in tissue architecture. Next, the indexical mode treats observed features as clues to underlying biological functions and pathologies. Finally, the symbolic mode integrates these observations into a shared vocabulary and diagnostic framework. Building on Peirce’s triad, Barthes’s denotation–connotation distinction, Goodman’s view of representation, and Merleau-Ponty’s notion of embodied perception, we show that understanding histology relies on learned visual codes and sensorimotor engagement. These insights inform pedagogical strategies—such as curated image sets, scaffolded inference tasks, explicit symbol teaching, and embodied exercises like gestural mapping and virtual reality—to guide students from initial confusion toward confident, fluent interpretation.</p>