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<p><i>Cestrum diurnum</i> is a medicinally important yet underexplored member of the Solanaceae family, known for its bioactive compounds such as terpenoids and alkaloids. Despite its pharmaceutical potential and toxicological concerns, the molecular insights into the specialized metabolite biosynthesis in this species have remained uncharacterized due to the absence of genomic resources. In this investigation, Illumina RNA-seq was employed to generate the first comprehensive transcriptome of <i>C. diurnum</i> leaves, coupled with metabolite profiling. The assembled transcriptome revealed complete sets of genes involved in terpenoid and alkaloid biosynthesis. Abundant transcription factors, including <i>MYB</i>, <i>bHLH</i>, and <i>WRKY</i> families, and highly connected protein-protein interaction modules suggested coordinated regulation of these pathways. Metabolite analysis confirmed the presence of pharmaceutical important triterpenoids such as oleanolic acid, ursolic acid, and β-amyrin, alongside alkaloids including nicotine and its derivatives. By integrating transcriptomic, regulatory, and metabolomic insights, this work provides a foundational resource for <i>C. diurnum.</i> It enables the discovery and functional characterization of candidate genes, supports metabolic engineering strategies to enhance valuable terpenoids, and offers avenues to mitigate toxic alkaloid accumulation. More extensively, these findings expand molecular knowledge of specialized metabolism in Solanaceae and unlock the potential of <i>C. diurnum</i> for future pharmaceutical and biotechnological applications.</p>

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Transcriptomic landscape of Cestrum diurnum: elucidating pathways of medicinally important terpenoids and toxic alkaloids

  • 期刊论文

  • 发表日期：

    2026年7月29日

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  • Vandana Mathur,
  • Ramawatar Nagar,
  • Maniraj Rathinam,
  • Shashank Kumar Mishra,
  • Bhanu Prakash,
  • Ajit Kumar Shasany,
  • Rohini Sreevathsa

摘要

Cestrum diurnum is a medicinally important yet underexplored member of the Solanaceae family, known for its bioactive compounds such as terpenoids and alkaloids. Despite its pharmaceutical potential and toxicological concerns, the molecular insights into the specialized metabolite biosynthesis in this species have remained uncharacterized due to the absence of genomic resources. In this investigation, Illumina RNA-seq was employed to generate the first comprehensive transcriptome of C. diurnum leaves, coupled with metabolite profiling. The assembled transcriptome revealed complete sets of genes involved in terpenoid and alkaloid biosynthesis. Abundant transcription factors, including MYB, bHLH, and WRKY families, and highly connected protein-protein interaction modules suggested coordinated regulation of these pathways. Metabolite analysis confirmed the presence of pharmaceutical important triterpenoids such as oleanolic acid, ursolic acid, and β-amyrin, alongside alkaloids including nicotine and its derivatives. By integrating transcriptomic, regulatory, and metabolomic insights, this work provides a foundational resource for C. diurnum. It enables the discovery and functional characterization of candidate genes, supports metabolic engineering strategies to enhance valuable terpenoids, and offers avenues to mitigate toxic alkaloid accumulation. More extensively, these findings expand molecular knowledge of specialized metabolism in Solanaceae and unlock the potential of C. diurnum for future pharmaceutical and biotechnological applications.