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<p>The cell-to-cell and systemic movement of cucumber mosaic virus (CMV) depends on its movement protein (MP) and 2b proteins, respectively, along with various host factors. In this study, we found that the small subunit of Rubisco (RBCS) associates with CMV MP and 2b protein of the subgroup II strain, as evaluated by yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. In BiFC, the interaction between MP-RBCS and MP-2b was mapped close to the cell borders as punctate spots. Similarly, the 2b-RBCS complex was observed near the cell borders as well as in the nucleus. Transient knockdown of RBCS in <i>Nicotiana benthamiana</i> using tobacco rattle virus-based virus-induced gene silencing (TRV-VIGS) resulted in the chlorosis of TRV2:RBCS plant leaves distinct from TRV2:00 control plants. Further, TRV2:RBCS plants exhibited a significant reduction in CMV accumulation compared to TRV2:00 control plants, highlighting the importance of RBCS in CMV infection. Overall, this study suggests that the association of CMV MP and 2b proteins with RBCS may influence viral accumulation and eventually its spread.</p>

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The Rubisco small subunit of cucumber interacts with the cucumber mosaic virus movement and silencing suppressor proteins

  • 期刊论文

  • 发表日期：

    2026年4月21日

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  • Reenu Kumari,
  • Surender Kumar,
  • Usha Kumari Rattan,
  • Rituraj Purohit,
  • Lakhmir Singh,
  • Vipin Hallan

摘要

The cell-to-cell and systemic movement of cucumber mosaic virus (CMV) depends on its movement protein (MP) and 2b proteins, respectively, along with various host factors. In this study, we found that the small subunit of Rubisco (RBCS) associates with CMV MP and 2b protein of the subgroup II strain, as evaluated by yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. In BiFC, the interaction between MP-RBCS and MP-2b was mapped close to the cell borders as punctate spots. Similarly, the 2b-RBCS complex was observed near the cell borders as well as in the nucleus. Transient knockdown of RBCS in Nicotiana benthamiana using tobacco rattle virus-based virus-induced gene silencing (TRV-VIGS) resulted in the chlorosis of TRV2:RBCS plant leaves distinct from TRV2:00 control plants. Further, TRV2:RBCS plants exhibited a significant reduction in CMV accumulation compared to TRV2:00 control plants, highlighting the importance of RBCS in CMV infection. Overall, this study suggests that the association of CMV MP and 2b proteins with RBCS may influence viral accumulation and eventually its spread.