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<p>The ERF (Ethylene Response Factor) transcription factor family is an important member of the <i>AP2/ERF</i> superfamily, playing a crucial role in plant growth and development, and responses to stresses. With the completion of the cucumber genome sequencing, a systematic analysis of the <i>CsERF</i> family has become possible. This study based on cucumber genome data, identified 127 <i>CsERFs</i> and divided them into 10 subgroups based on their phylogenetic relationships. Subsequently, the genes were analyzed for structure, chromosome localization, and physicochemical properties. Analysis of <i>cis</i>-elements revealed that most promoters of the <i>CsERFs</i> contain plant hormone response elements and stress response elements (such as those for auxin, abscisic acid, and defense and stress). The tissue-specific expression patterns of these genes were also examined, showing that they are expressed in various tissues. Under low-temperature treatment, the expression of <i>CsERF017</i> and <i>CsERF061</i> was significantly induced. Overexpression of <i>35S:CsERF017</i> and <i>35S:CsERF061</i> in cucumbers significantly enhanced cold tolerance, indicating their role in the cold signaling pathway of cucumbers. These findings provide insights into the molecular mechanisms by which <i>ERF</i> genes regulate cold tolerance in cucumbers and offer important evidence for genetic improvement of cucumber stress tolerance.</p>

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Comprehensive analysis of cucumber ERF family genes and functional characterization of CsERF017 and CsERF061 in cold tolerance in cucumber

  • 期刊论文

  • 发表日期：

    2026年1月26日

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  • Jingdan Zhang,
  • Chenyang Yue,
  • Huiwen Wang,
  • Wenxing He,
  • Hongmei Li,
  • Jialin Li

摘要

The ERF (Ethylene Response Factor) transcription factor family is an important member of the AP2/ERF superfamily, playing a crucial role in plant growth and development, and responses to stresses. With the completion of the cucumber genome sequencing, a systematic analysis of the CsERF family has become possible. This study based on cucumber genome data, identified 127 CsERFs and divided them into 10 subgroups based on their phylogenetic relationships. Subsequently, the genes were analyzed for structure, chromosome localization, and physicochemical properties. Analysis of cis-elements revealed that most promoters of the CsERFs contain plant hormone response elements and stress response elements (such as those for auxin, abscisic acid, and defense and stress). The tissue-specific expression patterns of these genes were also examined, showing that they are expressed in various tissues. Under low-temperature treatment, the expression of CsERF017 and CsERF061 was significantly induced. Overexpression of 35S:CsERF017 and 35S:CsERF061 in cucumbers significantly enhanced cold tolerance, indicating their role in the cold signaling pathway of cucumbers. These findings provide insights into the molecular mechanisms by which ERF genes regulate cold tolerance in cucumbers and offer important evidence for genetic improvement of cucumber stress tolerance.