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<p>The whitefly <i>Bemisia tabaci</i> poses a significant threat to agriculture by transmitting various plant viruses, being globally invasive and polyphagous in nature. For the management of this pest, various cultural and chemical methods are employed, but challenges such as insecticide resistance and environmental impact have made management of whiteflies difficult, which has led to the exploration of RNA interference (RNAi) as a sustainable and precise alternative. RNAi using double-stranded RNA (dsRNA) offers a promising, species-specific strategy for whitefly management. However, the naked dsRNA delivered exogenously on plant surfaces is unstable in nature, which limits its practical application. This study explores the use of Polyamidoamine dendrimer generation 5 (PAMAM) functionalized multiwalled carbon nanotubes (MWCNT) as a delivery vehicle to enhance dsRNA uptake. dsRNAs targeting the <i>B. tabaci</i>, Vitellogenin receptor (<i>BtVgR</i>) and Ryanodine receptor gene (<i>BtRyR</i>), designed to minimize off-target effects, were applied using the root dip method. To improve the biocompatibility and loading efficiency of carbon nanotubes, they were functionalized with PAMAM. In case of <i>BtRyR</i>, on day 7th, 83% mortality was observed at 80&#xa0;µg/mL concentration of dsRNA loaded on CNT, and for dsRNA alone, mortality observed on the 7th day was 80%. In case of <i>BtVgR</i>, on day 7, a slightly higher mortality of 69% was observed with the dsRNA-CNT complex compared to 66% with dsRNA alone at a concentration of 80&#xa0;µg/mL, indicating a modest improvement in delivery efficiency through CNTs. This study demonstrates that CNT-assisted RNAi targeting of <i>BtVgR</i>,<i> BtRyR</i> genes can serve as an efficient and environmentally friendly strategy for whitefly management.</p>

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RNAi-mediated silencing of Vitellogenin receptor and Ryanodine receptor, key genes, by using carbon nanotubes for management of whitefly (Bemisia tabaci)

  • 期刊论文

  • 发表日期：

    2026年2月17日

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  • Ankit Kumari,
  • Archna Suhag,
  • Yachna Jaiwal,
  • Manish Sainger,
  • Ranjana Jaiwal,
  • Pawan K. Jaiwal,
  • Darshna Chaudhary

摘要

The whitefly Bemisia tabaci poses a significant threat to agriculture by transmitting various plant viruses, being globally invasive and polyphagous in nature. For the management of this pest, various cultural and chemical methods are employed, but challenges such as insecticide resistance and environmental impact have made management of whiteflies difficult, which has led to the exploration of RNA interference (RNAi) as a sustainable and precise alternative. RNAi using double-stranded RNA (dsRNA) offers a promising, species-specific strategy for whitefly management. However, the naked dsRNA delivered exogenously on plant surfaces is unstable in nature, which limits its practical application. This study explores the use of Polyamidoamine dendrimer generation 5 (PAMAM) functionalized multiwalled carbon nanotubes (MWCNT) as a delivery vehicle to enhance dsRNA uptake. dsRNAs targeting the B. tabaci, Vitellogenin receptor (BtVgR) and Ryanodine receptor gene (BtRyR), designed to minimize off-target effects, were applied using the root dip method. To improve the biocompatibility and loading efficiency of carbon nanotubes, they were functionalized with PAMAM. In case of BtRyR, on day 7th, 83% mortality was observed at 80 µg/mL concentration of dsRNA loaded on CNT, and for dsRNA alone, mortality observed on the 7th day was 80%. In case of BtVgR, on day 7, a slightly higher mortality of 69% was observed with the dsRNA-CNT complex compared to 66% with dsRNA alone at a concentration of 80 µg/mL, indicating a modest improvement in delivery efficiency through CNTs. This study demonstrates that CNT-assisted RNAi targeting of BtVgR, BtRyR genes can serve as an efficient and environmentally friendly strategy for whitefly management.