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<p>The light-harvesting chlorophyll a/b-binding proteins (Lhcb) are an essential component of the photosynthetic antenna system, playing a critical role in both photosynthesis and the regulation of plant stress responses. In the rapeseed (<i>Brassica napus</i> L.) genome, we identified eight <i>BnaLhcb</i> genes, which were phylogenetically classified into three distinct groups. These genes exhibited a significant level of structural conservation and were distributed across ten chromosomes. Given the established role of <i>Lhcb</i> genes in abiotic stress defense, we investigated their response to chromium (Cr) stress in both Cr-tolerant and Cr-sensitive rapeseed cultivars. Notably, the expression of <i>BnaLhcb5.3</i> was significantly downregulated in the sensitive cultivar and upregulated in the tolerant one, indicating its potential role in Cr stress adaptation. The <i>BnaLhcb5.3</i> cDNA was successfully cloned, and its subcellular localization was confirmed to be within the chloroplast. Functional characterization using transgenic <i>Arabidopsis</i> plants overexpressing <i>BnaLhcb5.3</i> demonstrated enhance in Cr tolerance, improved plant growth, and biosynthesis of photosynthetic pigments. These plants also exhibited superior gas exchange parameters, higher activities of photosystem I (PSI) and photosystem II (PSII), and reduced ROS accumulation due to a strengthened antioxidant enzymatic defense system under Cr stress. Overall, our results demonstrated that <i>BnaLhcb5.3</i> plays a vital role in modulating growth responses and is a key factor in enhancing Cr tolerance in rapeseed.</p>

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Overexpression of rapeseed BnaLhcb5.3 mediates cellular metabolism and antioxidant defense under chromium stress

  • 期刊论文

  • 发表日期：

    2025年11月4日

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  • Iram Batool,
  • Tongjun Qin,
  • Ahsan Ayyaz,
  • Fakhir Hannan,
  • Muhammad Javed,
  • Weiqi Chen,
  • Zafar Ullah Zafar,
  • Muhammad Shahbaz Naeem,
  • Muhammad Ahsan Farooq,
  • Weijun Zhou

摘要

The light-harvesting chlorophyll a/b-binding proteins (Lhcb) are an essential component of the photosynthetic antenna system, playing a critical role in both photosynthesis and the regulation of plant stress responses. In the rapeseed (Brassica napus L.) genome, we identified eight BnaLhcb genes, which were phylogenetically classified into three distinct groups. These genes exhibited a significant level of structural conservation and were distributed across ten chromosomes. Given the established role of Lhcb genes in abiotic stress defense, we investigated their response to chromium (Cr) stress in both Cr-tolerant and Cr-sensitive rapeseed cultivars. Notably, the expression of BnaLhcb5.3 was significantly downregulated in the sensitive cultivar and upregulated in the tolerant one, indicating its potential role in Cr stress adaptation. The BnaLhcb5.3 cDNA was successfully cloned, and its subcellular localization was confirmed to be within the chloroplast. Functional characterization using transgenic Arabidopsis plants overexpressing BnaLhcb5.3 demonstrated enhance in Cr tolerance, improved plant growth, and biosynthesis of photosynthetic pigments. These plants also exhibited superior gas exchange parameters, higher activities of photosystem I (PSI) and photosystem II (PSII), and reduced ROS accumulation due to a strengthened antioxidant enzymatic defense system under Cr stress. Overall, our results demonstrated that BnaLhcb5.3 plays a vital role in modulating growth responses and is a key factor in enhancing Cr tolerance in rapeseed.