Scholarlink

<p>Nitrate transporters play important roles in nitrogen (N) uptake and utilization in plants. The function of nitrate transporter 2 (NRT2) in model plants under low-N (LN) conditions has been studied, but there are few studies on non-model plants, including Tartary buckwheat (an important medicinal and edible crop). In this study, seven <i>NRT2</i> genes were identified in Tartary buckwheat genome. All the FtNRT2 proteins were localized to the cell membrane with 10–12 transmembrane domains, and have the common structural characteristics of NRT2. Expression analysis showed <i>FtNRT2.1</i> was expressed in all tissues, <i>FtNRT2.3/2.4</i> were specifically expressed in roots, and <i>FtNRT2.6/2.7</i> were specifically expressed in seeds. Under LN, the expression of <i>FtNRT2.1/2.4</i> was induced, while <i>FtNRT2.3</i> was suppressed. The root-specific expressed gene <i>FtNRT2.4</i> may be the key NRT2 member for regulating LN response by sequence, molecular docking, and expression analysis. Overexpression of <i>FtNRT2.4</i> in tobacco improved plant growth and N uptake under 0 and 5&#xa0;mM N conditions. An ancillary protein of FtNRT2.4, FtNRT3.2, was characterized by yeast two-hybrid and firefly luciferase complementation assays. In addition, 14 transcription factors (TFs) may involve in the regulation of <i>FtNRT2.4</i> expression by co-expression analysis. FtNF-YB8, a TF localized in cytoplasm and nucleus, can bind to the promoter of <i>FtNRT2.4</i> by yeast one-hybrid analysis. Dual-luciferase reporter analysis showed that <i>FtNF-YB8</i> improved the expression of <i>FtNRT2.4</i>. These findings indicated the important role of <i>FtNRT2.4</i> in LN response and provide new insights into the regulatory function of NRT2.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genome-wide identification and expression analysis of NRT2 gene family in Tartary buckwheat suggests the potential role of FtNTR2.4 in low nitrogen response

  • 期刊论文

  • 发表日期：

    2025年10月11日

查看全文
  • Daiying Xu,
  • Yaoxuan Zou,
  • Nan Hu,
  • Han Li,
  • Junjie Yin,
  • Jiting Wang,
  • Xi Wu,
  • Dabing Xiang,
  • Jianglin Zhao,
  • Xiaoqin Zheng,
  • Yan Wan,
  • Yanxia Liu,
  • Changying Liu

摘要

Nitrate transporters play important roles in nitrogen (N) uptake and utilization in plants. The function of nitrate transporter 2 (NRT2) in model plants under low-N (LN) conditions has been studied, but there are few studies on non-model plants, including Tartary buckwheat (an important medicinal and edible crop). In this study, seven NRT2 genes were identified in Tartary buckwheat genome. All the FtNRT2 proteins were localized to the cell membrane with 10–12 transmembrane domains, and have the common structural characteristics of NRT2. Expression analysis showed FtNRT2.1 was expressed in all tissues, FtNRT2.3/2.4 were specifically expressed in roots, and FtNRT2.6/2.7 were specifically expressed in seeds. Under LN, the expression of FtNRT2.1/2.4 was induced, while FtNRT2.3 was suppressed. The root-specific expressed gene FtNRT2.4 may be the key NRT2 member for regulating LN response by sequence, molecular docking, and expression analysis. Overexpression of FtNRT2.4 in tobacco improved plant growth and N uptake under 0 and 5 mM N conditions. An ancillary protein of FtNRT2.4, FtNRT3.2, was characterized by yeast two-hybrid and firefly luciferase complementation assays. In addition, 14 transcription factors (TFs) may involve in the regulation of FtNRT2.4 expression by co-expression analysis. FtNF-YB8, a TF localized in cytoplasm and nucleus, can bind to the promoter of FtNRT2.4 by yeast one-hybrid analysis. Dual-luciferase reporter analysis showed that FtNF-YB8 improved the expression of FtNRT2.4. These findings indicated the important role of FtNRT2.4 in LN response and provide new insights into the regulatory function of NRT2.