Scholarlink

<p>This study investigated the molecular features and gene expression of tomato sucrose transporters (<i>SUTs</i>) in response to water deficit and the effects of ripening mutations <i>rin</i> and <i>Nr</i>. In silico analyses were carried out to characterize the amino acid sequences, conserved domains and gene structure of the <i>SlSUTs</i>. Wild-type (<i>WT</i>) and two near isogenic lines (NILs) of Micro-Tom harbouring the <i>rin</i> and <i>Nr</i> mutations were subjected to control and water deficit treatments and physiological and molecular analyses were carried out, including leaf gas exchange, antioxidant enzyme activity, soluble sugars concentration, and <i>SUT</i> gene expression in different source-sink organs. The results showed that the <i>SlSUT</i> genes are structurally conserved but variable in sequences, suggesting functional specialization within this gene family. Plant phenotyping revealed a metabolic adjustment of tomato plants grown under water deficit, including an increase in the concentration of soluble sugars in fruits and leaves. <i>SlSUT1</i> and <i>SlSUT4</i> were responsive to water deficit mainly in leaves and fruits, with such responses being annulled in leaves by the <i>rin</i> mutation. In addition, <i>SlSUT4</i> was down-regulated by water deficit in roots, irrespective of the genotype, and showed a co-regulated expression with <i>SlETR2</i>. <i>SlSUT2</i> was also induced by water-deficit in leaves and fruits, with the <i>Nr</i> mutation making it responsive also in roots. Collectively, these data indicate that <i>SlSUT</i> genes are structurally conserved but functionally distinct, exhibiting a differentially regulated expression in response to water deficit and <i>RIN</i> and <i>Nr</i> signaling in different source-sink organs.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Molecular characterization and expression of sucrose transporters (SUTs) in response to water deficit in tomato and effects of Rin and Nr mutations

  • 期刊论文

  • 发表日期：

    2025年10月11日

查看全文
  • Ana C. O. Barbosa,
  • Glaucia C. B. Silva,
  • Dilson S. Rocha Jr.,
  • Maria L. do C. Santos,
  • Paulo H. G. A. de Oliveira,
  • Aline A. Cavalari,
  • Marcio G. C. Costa

摘要

This study investigated the molecular features and gene expression of tomato sucrose transporters (SUTs) in response to water deficit and the effects of ripening mutations rin and Nr. In silico analyses were carried out to characterize the amino acid sequences, conserved domains and gene structure of the SlSUTs. Wild-type (WT) and two near isogenic lines (NILs) of Micro-Tom harbouring the rin and Nr mutations were subjected to control and water deficit treatments and physiological and molecular analyses were carried out, including leaf gas exchange, antioxidant enzyme activity, soluble sugars concentration, and SUT gene expression in different source-sink organs. The results showed that the SlSUT genes are structurally conserved but variable in sequences, suggesting functional specialization within this gene family. Plant phenotyping revealed a metabolic adjustment of tomato plants grown under water deficit, including an increase in the concentration of soluble sugars in fruits and leaves. SlSUT1 and SlSUT4 were responsive to water deficit mainly in leaves and fruits, with such responses being annulled in leaves by the rin mutation. In addition, SlSUT4 was down-regulated by water deficit in roots, irrespective of the genotype, and showed a co-regulated expression with SlETR2. SlSUT2 was also induced by water-deficit in leaves and fruits, with the Nr mutation making it responsive also in roots. Collectively, these data indicate that SlSUT genes are structurally conserved but functionally distinct, exhibiting a differentially regulated expression in response to water deficit and RIN and Nr signaling in different source-sink organs.