Scholarlink

<p>Zinc oxide nanoparticles (ZnO-NPs) have recently been used to alleviate arsenic (As) phytotoxicity in crops. However, no comparative research has assessed the potential of chemically and biologically synthesized ZnO-NPs in wheat varieties under As stress. Therefore, the present study conducted a comparative assessment of two ZnO-NPs to alleviate As stress in two wheat varieties (BARANI-70 and NARC-2009). In a hydroponic experiment (28–30&#xa0;days), varieties were exposed to arsenate stress (200 uM/L), chemically synthesized ZnO-NPs, and biologically synthesized ZnO-NPs (100&#xa0;mg/L and 200&#xa0;mg/L). The present study performed histological analyses to assess the role of ZnO-NPs in alleviating As stress at the cellular level. Furthermore, the quantification of zinc (Zn) and As was carried out in wheat tissues. The present research examined the activities of antioxidant enzymes and secondary metabolites in alleviating As-induced oxidative stress by ZnO-NPs. Chemically synthesized NPs (100&#xa0;mg/L) lowered oxidative stress markers (MDA, H<sub>2</sub>O<sub>2</sub>) while balancing pyruvate and GSH content in roots and shoots of NARC-2009. On the contrary, such particles at 200&#xa0;mg/L increased oxidative stress in both varieties, prominently in Barani-70. Biogenic ZnO-NPs reduced oxidative stress in roots and shoots of both varieties, at both 100&#xa0;mg/L and 200&#xa0;mg/L exposures. Increased stele thickness and reduced cortex thickness were observed under chemically synthesized NPs, while biogenic NPs restored normal root anatomy in both varieties. Chemically synthesized NPs increased Zn accumulation and reduced As in shoots and roots, but biogenic NPs achieved similar As mitigation with balanced Zn levels. Lower antioxidant activities and metabolites (flavonoids, phenols, proline) indicated reduced stress with biogenic NPs. The findings revealed that biogenic ZnO-NPs at 100&#xa0;mg/L were less toxic and more effective for As stress alleviation in both wheat varieties.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Unraveling the potential of zinc oxide nanoparticles on alleviating arsenic toxicity in wheat (Triticum aestivum L.) varieties: a comparative assessment of histology and phenomics

  • 期刊论文

  • 发表日期：

    2025年5月16日

查看全文
  • Eimaan Aamer,
  • Muhammad Saeed,
  • Faroha Liaqat,
  • Muhammad Ramzan Khan,
  • Muhammad Kashif Naeem,
  • Ghazala Mustafa,
  • Riffat Naseem Malik

摘要

Zinc oxide nanoparticles (ZnO-NPs) have recently been used to alleviate arsenic (As) phytotoxicity in crops. However, no comparative research has assessed the potential of chemically and biologically synthesized ZnO-NPs in wheat varieties under As stress. Therefore, the present study conducted a comparative assessment of two ZnO-NPs to alleviate As stress in two wheat varieties (BARANI-70 and NARC-2009). In a hydroponic experiment (28–30 days), varieties were exposed to arsenate stress (200 uM/L), chemically synthesized ZnO-NPs, and biologically synthesized ZnO-NPs (100 mg/L and 200 mg/L). The present study performed histological analyses to assess the role of ZnO-NPs in alleviating As stress at the cellular level. Furthermore, the quantification of zinc (Zn) and As was carried out in wheat tissues. The present research examined the activities of antioxidant enzymes and secondary metabolites in alleviating As-induced oxidative stress by ZnO-NPs. Chemically synthesized NPs (100 mg/L) lowered oxidative stress markers (MDA, H2O2) while balancing pyruvate and GSH content in roots and shoots of NARC-2009. On the contrary, such particles at 200 mg/L increased oxidative stress in both varieties, prominently in Barani-70. Biogenic ZnO-NPs reduced oxidative stress in roots and shoots of both varieties, at both 100 mg/L and 200 mg/L exposures. Increased stele thickness and reduced cortex thickness were observed under chemically synthesized NPs, while biogenic NPs restored normal root anatomy in both varieties. Chemically synthesized NPs increased Zn accumulation and reduced As in shoots and roots, but biogenic NPs achieved similar As mitigation with balanced Zn levels. Lower antioxidant activities and metabolites (flavonoids, phenols, proline) indicated reduced stress with biogenic NPs. The findings revealed that biogenic ZnO-NPs at 100 mg/L were less toxic and more effective for As stress alleviation in both wheat varieties.