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<p><i>CEN-like protein 2</i> of pigeon pea, a candidate anti-florigen gene and a close homolog of <i>AtTFL1</i> (<i>Arabidopsis Terminal Flower1</i>) of the PEBP family has been characterized through constitutive expression in tobacco. <i>In-silico</i> analysis helped to demonstrate the absence of a nuclear binding domain and the conserveness of substrate binding sites of this protein across angiosperms. Transgenic tobacco lines with 2–eightfold higher expressions of <i>CEN-like protein 2</i> showed delayed flowering (26–32&#xa0;days) along with significant morphological changes, including vegetative vigour, number and size of flowers, fruit setting, etc. Together, these findings showed that <i>CEN-like protein 2</i> not only delays floral transition through repression but also regulates a variety of developmental traits. Expression profiling of upstream and downstream interacting pathway genes explained that their expression modulation led to a prolonged vegetative phase of over-expressed lines. Floral inducer genes like <i>APETALA1</i> and <i>LEAFY</i> were drastically down-regulated in transgenic lines, reconfirming the role of the <i>CEN-like 2</i> gene in floral regulation. In conclusion, precisely controlling <i>CcCEN-like 2</i> gene expression may prove useful for refining pigeon pea breeding.</p>

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Constitutive expression of CEN-like protein 2, a TFL1 ortholog of pigeon pea (Cajanus cajan [L.] Millspaugh) delays flowering in transgenic tobacco plants

  • 期刊论文

  • 发表日期：

    2025年3月19日

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  • Sougata Bhattacharjee,
  • Krishnayan Paul,
  • K. Venkat Raman,
  • Jyotsana Tilgam,
  • Priyanka Kumari,
  • Mahi Baaniya,
  • Rohini Sreevathsa,
  • Anjali Anand,
  • G. Rama Prashat,
  • Debasis Pattanayak

摘要

CEN-like protein 2 of pigeon pea, a candidate anti-florigen gene and a close homolog of AtTFL1 (Arabidopsis Terminal Flower1) of the PEBP family has been characterized through constitutive expression in tobacco. In-silico analysis helped to demonstrate the absence of a nuclear binding domain and the conserveness of substrate binding sites of this protein across angiosperms. Transgenic tobacco lines with 2–eightfold higher expressions of CEN-like protein 2 showed delayed flowering (26–32 days) along with significant morphological changes, including vegetative vigour, number and size of flowers, fruit setting, etc. Together, these findings showed that CEN-like protein 2 not only delays floral transition through repression but also regulates a variety of developmental traits. Expression profiling of upstream and downstream interacting pathway genes explained that their expression modulation led to a prolonged vegetative phase of over-expressed lines. Floral inducer genes like APETALA1 and LEAFY were drastically down-regulated in transgenic lines, reconfirming the role of the CEN-like 2 gene in floral regulation. In conclusion, precisely controlling CcCEN-like 2 gene expression may prove useful for refining pigeon pea breeding.