<p>Vitamin C, scientifically known as ascorbic acid, is structurally related to glucose. The physical, biological, and chemical functions of Vitamin C are due to its electron-donor nature. Ascorbate readily undergoes autoxidation, generating hydrogen peroxide at a specific pH. In vitro studies reveal that Vitamin C functions as an antioxidant at low concentrations; however, it is pro-oxidant at high concentrations. Thus, ascorbic acid has cytotoxic effects on cancer cells. Clinical trials evaluating high-dose intravenous L-ascorbic acid have yielded mixed and, in some cases, inconclusive results. This review article covers the following aspects related to Vitamin C, such as the significance and metabolism of Vitamin C, association of Vitamin C with various types of cancer, epigenetic treatment and role of Vitamin C in epigenetic, recent clinical and preclinical studies related to Vitamin C, Anti-carcinogenic mechanism of Vitamin C, safety and toxicity associated with ascorbic acid followed by concluding remarks.</p> Graphical abstract <p></p>