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<p>Evidence suggests that downregulation of miR-153 in hepatocellular carcinoma (HCC) cells and tissues promotes cell proliferation. One of the characteristics of cancer cells is the high expression of B-cell lymphoma 2 (<i>BCL2</i>) gene inside the cells, which is a barrier to cell apoptosis. In this study, cloned miR-153 transfection into the HepG2 cancer cells altered the expression of genes associated with autophagy, reduced the <i>BCL2</i> gene expression, and resulted in apoptosis, as assessed by real-time PCR. The BCL2 gene expression decreased significantly, <i>P</i> &lt; 0.01, in the miR-153-transfected cells, compared to the negative control and empty vector-transfected cells. This analysis was performed using real-time PCR, and its comparison was evaluated using Prism software. This reduction disrupted the Beclin 1 and BCL2 complexes as a type of signal to initiate the process. Also, the expression of <i>light chain 3</i> and <i>BECN1</i> genes increased as autophagic genes. The cloning of miR-153 in the PLKO1 vector was found and to be effective, and the expression of miR-153 was confirmed inside the target cells. By targeting the <i>BCL2</i> gene, reducing its expression, and increasing the expression of <i>LC3</i> and <i>BECN1</i> genes in cancer cells, miR-153 might cause apoptosis and autophagy within the HCC cell line. The luciferase assay was used for gene targeting and results was analyzed by ANOVA.</p>

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miR-153 Overexpression and BCL2 Targeting Induce Apoptosis and Autophagy in HepG2 Cells

  • 期刊论文

  • 发表日期：

    2025年9月22日

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  • Samira Nafar,
  • Jafar Fallahi,
  • Sepideh Moradi,
  • Gholamhossein Tamaddon,
  • Seyed Mohammad Bagher Tabei

摘要

Evidence suggests that downregulation of miR-153 in hepatocellular carcinoma (HCC) cells and tissues promotes cell proliferation. One of the characteristics of cancer cells is the high expression of B-cell lymphoma 2 (BCL2) gene inside the cells, which is a barrier to cell apoptosis. In this study, cloned miR-153 transfection into the HepG2 cancer cells altered the expression of genes associated with autophagy, reduced the BCL2 gene expression, and resulted in apoptosis, as assessed by real-time PCR. The BCL2 gene expression decreased significantly, P < 0.01, in the miR-153-transfected cells, compared to the negative control and empty vector-transfected cells. This analysis was performed using real-time PCR, and its comparison was evaluated using Prism software. This reduction disrupted the Beclin 1 and BCL2 complexes as a type of signal to initiate the process. Also, the expression of light chain 3 and BECN1 genes increased as autophagic genes. The cloning of miR-153 in the PLKO1 vector was found and to be effective, and the expression of miR-153 was confirmed inside the target cells. By targeting the BCL2 gene, reducing its expression, and increasing the expression of LC3 and BECN1 genes in cancer cells, miR-153 might cause apoptosis and autophagy within the HCC cell line. The luciferase assay was used for gene targeting and results was analyzed by ANOVA.