<p>Platelet transfusions are essential for patients at risk of spontaneous bleeding. Single donor platelet (SDP) transfusions limit donor exposure and, at the same time, provide a quality platelet product that may have a significant impact on patient outcomes. This study aimed to identify donor variables that would affect the platelet yield of SDP products. In the present study, 161 SDP procedures that were performed between July 2021 and December 2021 on three different cell separator platforms were included. Donor variables(age, weight, hemoglobin, packed cell volume (PCV), platelet count, platelet crit (PCT), and platelet distribution width (PDW)), product variables (unit volume, platelet count, platelet yield, red blood cell (RBC) count, PCT, and PDW) and patient pre- and 24&#xa0;h post-transfusion platelet counts were obtained. Correlation with the yield of the SDP product donor variables and patient platelet increment was then analyzed. The mean yield of platelets in the SDP product was 4.08 ± 0.68 × 10¹¹ per unit. Platelet count (283.27 ± 58.67 × 10³/µL), PCT (0.24 ± 0.05%), and PDW (14.78 ± 0.69%) showed significant correlation with yield, though this association was not significant in the multivariate analysis. Other variables did not show a significant correlation with product yield. There was no relationship between the SDP yield and patient platelet increment. This study showed the pre-donation platelet count, PCT, and PDW of donors influenced the yield of SDP by univariate analysis. Larger prospective multicentric studies focusing on factors affecting the yield of a product would help in predicting factors to obtain a better yield of the product.</p>