Random Donor Platelet Concentrate’s Quality Analysis: Overnight Holding Effects of Whole Blood and Buffy Coat
摘要
Blood component therapy serves as a primary modality in transfusion practices. However, the regulatory requirement of 6 h holding time of whole blood (WB) at room temperatures (RT), restricts the overall capacity for component separation. The aim of this study was to evaluate the quality of the blood components specifically random donor platelets (RDP) prepared from WB after extended holding times. A prospective experimental study involving three distinct groups (1) Control group: WB processed after a standard hold time of 6 h at RT, (2) Overnight whole blood (OWB) group: WB processed after 18–24 h hold at RT and (3) Overnight buffy coat (OBC) group: RDPs prepared after 18–24 h hold of buffy coat at RT. Basic biochemical and quality parameters, as per national standards were evaluated and compared for all the components. All blood components in three groups were within normal limit of quality parameters. However, RDPs in OWB group had lower pH and glucose and higher MPV levels, whereas in OBC group lower pH, and higher glucose and PaCO2 levels at the end of storage. Both the test groups had lower pH (6.9 in OWB and OBC vs 7.2 in control) and higher PaCO2 (66.94 mmHg in OWB & 44 mmHg in OBC vs 41.9 mmHg in control), and glucose levels (306.3 mg/dl in OWB & 306 mg/dl in OBC vs 370.3 mg/dl in control). Components prepared from all three methods had almost similar quality parameters and coincide within national standards. Either of these methods can be used for better utilisation of resources.