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<p>An qPCR-based workflow for Mycoplasma detection was evaluated under defined matrix conditions using HEK cell suspensions spiked with low concentrations of relevant Mycoplasma species. The assay combined sensitive target amplification with an internal control for monitoring extraction efficiency and amplification performance. The results demonstrate that qPCR-based methods enable rapid and sensitive Mycoplasma detection and represent a suitable alternative to classical culture-based approaches for routine testing in regulated environments.</p>

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Sensitiver Nachweis von Mykoplasmen in Zellkulturen mittels qPCR

  • 期刊论文

  • 发表日期：

    2026年6月18日

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  • Ines Leonhardt,
  • Michel Gerke

摘要

An qPCR-based workflow for Mycoplasma detection was evaluated under defined matrix conditions using HEK cell suspensions spiked with low concentrations of relevant Mycoplasma species. The assay combined sensitive target amplification with an internal control for monitoring extraction efficiency and amplification performance. The results demonstrate that qPCR-based methods enable rapid and sensitive Mycoplasma detection and represent a suitable alternative to classical culture-based approaches for routine testing in regulated environments.