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<p>2D organoid monolayers cultured on Transwells enable polarized growth and recapitulate the cellular diversity and barrier function characteristics of the human duodenum. The common practice of pre-coating the Transwells with basement membrane extract (BME) is not necessary and instead, adds an additional diffusion barrier. BME-free 2D organoid models are more physiologically relevant for studying intestinal barrier function and help to reduce animal-derived materials in line with the 3R principle.</p>

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Matrix-freies 2D-Organoidmodell zur Erforschung der Darmbarrierefunktion

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月18日

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  • Nina A. Hering,
  • Kopano Valerie Masete,
  • Hans-Jörg Epple

摘要

2D organoid monolayers cultured on Transwells enable polarized growth and recapitulate the cellular diversity and barrier function characteristics of the human duodenum. The common practice of pre-coating the Transwells with basement membrane extract (BME) is not necessary and instead, adds an additional diffusion barrier. BME-free 2D organoid models are more physiologically relevant for studying intestinal barrier function and help to reduce animal-derived materials in line with the 3R principle.