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<p>Many cellular processes are observed by light microscopy, whereas ultrastructure requires electron microscopy (EM). Correlative light and electron microscopy (CLEM) combines functional fluorescence signals with EM ultrastructural resolution in the same sample, enabling precise correlation. We present an optimized CLEM workflow for plant samples combining high-pressure freezing, freeze substitution, confocal and EM to analyze the function and structure of dynamic endomembrane compartments.</p>

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Korrelative Mikroskopie: Funktion und Struktur in einer Methode vereint

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月25日

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  • Elsa Arcalís,
  • Sonja Huber,
  • Fabian Schubert,
  • Eva Stöger

摘要

Many cellular processes are observed by light microscopy, whereas ultrastructure requires electron microscopy (EM). Correlative light and electron microscopy (CLEM) combines functional fluorescence signals with EM ultrastructural resolution in the same sample, enabling precise correlation. We present an optimized CLEM workflow for plant samples combining high-pressure freezing, freeze substitution, confocal and EM to analyze the function and structure of dynamic endomembrane compartments.