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<p>Inspired by natural gene transfer systems, we developed ACTIMOT, a CRISPR-Cas9–based <i>in vivo</i> DNA mobilization and multiplication platform that transfers large biosynthetic gene clusters (BGCs) from bacterial chromosomes onto replicative plasmids within the same cell. This strategy amplifies and activates cryptic BGCs efficiently, greatly enhancing product yields and enabling the discovery of four new natural product families, thereby unlocking hidden microbial biosynthetic potential.</p>

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Naturinspirierte Strategie zur Expression ungenutzter Biosynthesewege

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月25日

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  • Feng Xie,
  • Rolf Müller,
  • Chengzhang Fu

摘要

Inspired by natural gene transfer systems, we developed ACTIMOT, a CRISPR-Cas9–based in vivo DNA mobilization and multiplication platform that transfers large biosynthetic gene clusters (BGCs) from bacterial chromosomes onto replicative plasmids within the same cell. This strategy amplifies and activates cryptic BGCs efficiently, greatly enhancing product yields and enabling the discovery of four new natural product families, thereby unlocking hidden microbial biosynthetic potential.