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<p>Much eukaryotic biodiversity, especially among fungi and microeukaryotes, remains unknown due to limited data and complex genomes. Standard metagenomics often fails with these “dark taxa.” A workflow using long-read sequencing, targeted primers, and laser microdissection enables genetic analysis of single cells. Tested on aquatic fungi, it yields barcodes and genomes, offering a new tool to reveal hidden microeukaryotic diversity by integrating microscopy and genomics.</p>

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Einzelzellgenomik für Biodiversitätsforschung von unbekannten Arten

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年9月4日

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  • Christian Wurzbacher

摘要

Much eukaryotic biodiversity, especially among fungi and microeukaryotes, remains unknown due to limited data and complex genomes. Standard metagenomics often fails with these “dark taxa.” A workflow using long-read sequencing, targeted primers, and laser microdissection enables genetic analysis of single cells. Tested on aquatic fungi, it yields barcodes and genomes, offering a new tool to reveal hidden microeukaryotic diversity by integrating microscopy and genomics.